Nemoto S, Koiso K, Aoyagi K, Tojo S
J Urol. 1985 Nov;134(5):996-8. doi: 10.1016/s0022-5347(17)47574-4.
Elastase activity in bladder cancer tissue and normal bladder epithelium was assayed with succinyl trialanine paranitroanilide (Suc-Ala3-NA), a synthetic substrate of elastase. Elastase in the tissue extract liberated paranitroanilide (NA) from Suc-Ala3-NA and coexistant endopeptidases produced alanine-NA and dialanine-NA. These products were detected by high performance liquid chromatography. All activities of elastase and endopeptidases in bladder cancer tissues from 20 patients are significantly higher than those in 17 normal bladder epithelia. In 20 bladder cancer cases evaluated, the relation between the activities of elastase and endopeptidases and the background factors of each bladder cancer case was examined. There was a significantly different activity of elastase between the superficial bladder cancer group (less than or equal to T1) and the invasive group (T2 less than or equal to). Elastase is assumed to play an important role in cancer invasion.
使用弹性蛋白酶的合成底物琥珀酰 - 丙氨酰 - 丙氨酰 - 对硝基苯胺（Suc - Ala3 - NA）测定膀胱癌组织和正常膀胱上皮中的弹性蛋白酶活性。组织提取物中的弹性蛋白酶从Suc - Ala3 - NA释放出对硝基苯胺（NA），共存的内肽酶产生丙氨酰 - NA和二丙氨酰 - NA。这些产物通过高效液相色谱法检测。20例膀胱癌组织中弹性蛋白酶和内肽酶的所有活性均显著高于17例正常膀胱上皮中的活性。在评估的20例膀胱癌病例中，研究了弹性蛋白酶和内肽酶活性与各膀胱癌病例背景因素之间的关系。浅表性膀胱癌组（小于或等于T1）和浸润性组（T2及以上）之间的弹性蛋白酶活性存在显著差异。推测弹性蛋白酶在癌症浸润中起重要作用。
