Croute F, Delaporte E, Bonnefoy J Y, Fertin C, Thivolet J, Nicolas J F
INSERM U.209, Hôpital E. Herriot, Lyon, France.
Br J Dermatol. 1991 Jun;124(6):538-41. doi: 10.1111/j.1365-2133.1991.tb04946.x.
We report the stimulation of elastase activity of human skin fibroblasts by interleukin-1 beta (IL-1). Elastase activity was determined by the hydrolysis of the synthetic substrate succinoyl trialanine paranitroanilide (Suc(Ala3)NA). Recombinant human IL-1 beta induced a concentration-dependent increase in the elastase activity of fibroblasts. The supernatants of keratinocyte cultures that contained IL-1/ETAF showed similar stimulatory activity.
我们报告了白细胞介素-1β（IL-1）对人皮肤成纤维细胞弹性蛋白酶活性的刺激作用。弹性蛋白酶活性通过合成底物琥珀酰-丙氨酰-丙氨酰-对硝基苯胺（Suc(Ala3)NA）的水解来测定。重组人IL-1β诱导成纤维细胞弹性蛋白酶活性呈浓度依赖性增加。含有IL-1/表皮细胞活化因子（ETAF）的角质形成细胞培养上清液显示出类似的刺激活性。
