Department of Functional Sciences, Faculty of Veterinary Medicine, Pharmacology and Toxicology, Fundamental and Applied Research for Animals & Health (FARAH), University of Liège, Liège 1 (Sart Tilman) 4000, Belgium.
Department of Functional Sciences, Faculty of Veterinary Medicine, Physiology and Sport Medicine, Fundamental and Applied Research for Animals & Health (FARAH), University of Liège, Liège 1 (Sart Tilman) 4000, Belgium.
Environ Toxicol Pharmacol. 2024 Sep;110:104515. doi: 10.1016/j.etap.2024.104515. Epub 2024 Jul 18.
Equine atypical myopathy (AM) is a severe rhabdomyolysis syndrome primarily caused by hypoglycin A (HGA) and methylenecyclopropylglycine protoxins. This study aimed to refine diagnostic and prognostic criteria for AM while exploring apparently healthy cograzers. Blood samples from 263 horses, including AM cases (n= 95), cograzers (n= 73), colic horses (n= 19), and controls (n= 76), were analyzed for HGA, its toxic metabolite, and acylcarnitines profile. Diseased horses exhibited alterations in acylcarnitines that strongly distinguished them from controls and colic horses. Regression analyses identified distinct acylcarnitines profiles among groups, with cograzers showing intermediate alterations. Age and gelding status emerged as protective factors against AM. Furthermore, serum acylcarnitines profiling was valuable in predicting AM survival, with isovaleryl-/2-methylbutyrylcarnitine (i.e., C5 acylcarnitine) showing promise as both a diagnostic and prognostic marker. Subclinical alterations in cograzers underscore a novel aspect: the presence of subclinical cases of AM.
马属动物非典型肌病（AM）是一种严重的横纹肌溶解综合征，主要由低血糖素 A（HGA）和亚甲基环丙基甘氨酸原毒素引起。本研究旨在完善 AM 的诊断和预后标准，并探讨看似健康的共 grazing 马。分析了 263 匹马的血液样本，包括 AM 病例（n=95）、共 grazing 马（n=73）、腹痛马（n=19）和对照马（n=76），以检测 HGA、其毒性代谢物和酰基辅酶 A 谱。患病马的酰基辅酶 A 谱发生改变，这使其与对照组和腹痛马明显区分开来。回归分析确定了不同组之间的独特酰基辅酶 A 谱，而共 grazing 马则显示出中间改变。年龄和去势状态是 AM 的保护因素。此外，血清酰基辅酶 A 谱分析在预测 AM 存活方面具有价值，异戊酰基/2-甲基丁酰基辅酶 A（即 C5 酰基辅酶 A）有望成为诊断和预后标志物。共 grazing 马的亚临床改变突显了一个新的方面：存在亚临床 AM 病例。
