Hosseini Elham, Aghajanpour Samaneh, Chekini Zahra, Zameni Nadia, Zolfaghary Zahra, Aflatoonian Reza, Hafezi Maryam
Zanjan Metabolic Diseases Research Centre, Zanjan University of Medical Sciences, Zanjan, Iran.
Department of Obstetrics and Gynaecology, Mousavi Hospital, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
Int J Fertil Steril. 2024 Jul 13;18(Suppl 1):22-29. doi: 10.22074/ijfs.2023.2000897.1461.
The follicular fluid (FF) of mature oocytes contains a high concentration of growth factors and cytokines that have the potential to influence implantation in either a paracrine or autocrine manner. During the physiological processes of ovulation, FF enters the fallopian tubes in conjunction with the oocyte. The purpose of this study is to evaluate implantation and clinical pregnancy rates following uterine flushing with FF and granulosa cells in infertile women with moderate male factor infertility after ovum retrieval for intracytoplasmic sperm injection (ICSI).
This phase III randomised clinical trial enrolled 140 women with moderate male factor infertility who intended to undergo ICSI at Royan Infertility Clinic (Tehran, Iran). A computer-generated program and opaque sealed envelopes were used to randomly allocate patients to either an intervention group (n=70) or a control group (n=70). Participants in the intervention group received 2 ml of clear FF (without blood contamination) from 2 to 3 dominant follicles after oocyte retrieval. The control group only underwent uterine cavity catheterisation.
The intervention group had a clinical pregnancy rate of 38.5% (25/65) compared to the control group [42.9% (27/63); P=0.719] and an implantation rate of 24.1% compared to the control group (27%; P=0.408). These rates did not differ between the groups. There were no statistically significant differences between the intervention and control groups in terms of pregnancy-related complications-ectopic pregnancy, blighted ovum or anembryonic pregnancy, and abortion.
Uterine cavity flushing with FF from mature follicles following oocyte retrieval had no effect, either positively or negatively, on clinical pregnancy or implantation rates in women with moderate male factor infertility (registration number: NCT04077970).
成熟卵母细胞的卵泡液（FF）含有高浓度的生长因子和细胞因子，它们有可能以旁分泌或自分泌方式影响着床。在排卵的生理过程中，卵泡液与卵母细胞一起进入输卵管。本研究的目的是评估在为进行卵胞浆内单精子注射（ICSI）而取卵后，对中度男性因素不育的不孕妇女进行卵泡液和颗粒细胞子宫冲洗后的着床率和临床妊娠率。
这项III期随机临床试验纳入了140名打算在伊朗德黑兰罗扬不孕不育诊所接受ICSI的中度男性因素不育妇女。使用计算机生成程序和不透明密封信封将患者随机分配到干预组（n = 70）或对照组（n = 70）。干预组的参与者在取卵后从2至3个优势卵泡中接受了2ml清亮的卵泡液（无血液污染）。对照组仅进行子宫腔插管。
干预组的临床妊娠率为38.
