Clinical Center Kragujevac, Clinic of Gynecology and Obstetrics, Department of Biomedically Assisted Fertilization, Kragujevac, Serbia.
University of Kragujevac, Faculty of Medical Sciences, PhD student, Kragujevac, Serbia.
Medicine (Baltimore). 2022 Jul 22;101(29):e29624. doi: 10.1097/MD.0000000000029624.
The quality of an oocyte is influenced by its microenvironment, which includes cumulus cells and follicular fluid, as well as cells of the immune system and their products. The ovarian interleukins, which are secreted by the granulosa cells and other immune cells within the ovaries and follicles, regulate various functions between the cells. IL-6 is a cytokine that is present in the follicular fluid and may affect the quality of oocytes. There are some inconsistencies in the literature regarding the concentration of interleukin 6 in the follicular fluid. The main objective of this study was to examine whether the concentration of interleukin 6 in the follicular fluid affects the outcome of IVF. This study involved 83 patients who underwent IVF. Follicular fluid was used as the biological material for the analysis. Examination of the obtained follicular fluid and collection of oocytes under a stereomicroscope was performed in the embryological laboratory. The concentration of IL-6 in the follicular fluid was analyzed. IVF and ICSI methods were used as the fertilization methods. Pregnancy was confirmed by the positive serum β-hCG level. The software package SPSS 20 was used for statistical data processing. Analysis of the follicular fluid samples showed a correlation between the concentration of IL-6 in the follicular fluid and the outcome of IVF. The concentration of IL-6 in the follicular fluid was higher in patients with confirmed pregnancy (9.55 ± 7.47 ng/ml). Based on our results, we conclude that the concentration of IL-6 affects the outcome of IVF. If the range of IL-6 concentration is between 3,67 ng/ml and 10 ng/ml, we can expect good IVF outcome with vital pregnancy.
卵母细胞的质量受到其微环境的影响，包括卵丘细胞和卵泡液，以及免疫系统细胞及其产物。卵巢白细胞介素由卵巢和卵泡中的颗粒细胞和其他免疫细胞分泌，调节细胞间的各种功能。IL-6 是一种细胞因子，存在于卵泡液中，可能影响卵母细胞的质量。关于卵泡液中白细胞介素 6 的浓度，文献中存在一些不一致之处。本研究的主要目的是研究卵泡液中白细胞介素 6 的浓度是否影响 IVF 的结局。这项研究涉及 83 名接受 IVF 的患者。卵泡液被用作分析的生物材料。在胚胎学实验室中，在立体显微镜下检查获得的卵泡液并收集卵母细胞。分析卵泡液中的 IL-6 浓度。使用 IVF 和 ICSI 方法作为受精方法。通过血清 β-hCG 水平阳性确认怀孕。使用 SPSS 20 软件包进行统计数据分析。对卵泡液样本的分析表明，卵泡液中 IL-6 的浓度与 IVF 的结局之间存在相关性。在确认怀孕的患者中，卵泡液中 IL-6 的浓度较高（9.55±7.47ng/ml）。基于我们的结果，我们得出结论，IL-6 的浓度会影响 IVF 的结局。如果 IL-6 浓度范围在 3.67ng/ml 和 10ng/ml 之间，我们可以预期良好的 IVF 结局和有活力的妊娠。
