Yadav Saroj Kumar, Shil Subrata Kumar, Pallab Monoar Sayeed, Islam Kh Nurul, Sutradhar Bibek Chandra, Das Bhajan Chandra
Department of Medicine and Surgery, Faculty of Veterinary Medicine, Chattogram Veterinary and Animal Sciences University, Chattogram, 4225, Bangladesh.
Department of Anatomy and Histology, Faculty of Veterinary Medicine, Chattogram veterinary and Animal Sciences University, Chattogram, 4225, Bangladesh.
Vet Anim Sci. 2024 Jun 21;25:100374. doi: 10.1016/j.vas.2024.100374. eCollection 2024 Sep.
Long bone fractures are common orthopedic conditions. There are numerous ways to repair these fractures. Bone grafting becomes necessary when a broken bone has a significant gap. However, due to insufficient donor volume and donor site morbidity, substitutes are required. In veterinary orthopaedics, calcium carbonate from cockle shells could be used as a bone biomaterial. We investigated its efficacy as a bone biomaterial repair for goat femoral fractures. The study included 10 healthy adult male Black Bengal goats weighing 8 kg and aged 12-13 months. The study includes control and treatment groups. Intramedullary pinning stabilized an 8-mm right femur diaphyseal fracture in the treatment and control groups. The treated group received 2 ml of bone paste in the fractured gap, whereas the control group left it empty. We examined all goats with X-rays on the 7th, 45th, and 60th days, followed by gross and histological findings. Due to callus bridging, radiographs revealed faster bone growth in the treated group than in the control group. Gross examination demonstrates the treated group had a larger fracture callus than the control group. Histopathology showed that bone formed faster and included more osteocytes, osteoblasts, osteoclasts, and bony spicules than in the control group. The treated group had more periosteum osteoblasts, while the control group had fibroblasts. These results showed that the treated group had more osteogenic activity than the control group. This study demonstrates the potential of cockle shell-based calcium carbonate bone paste as a synthetic biomaterial for healing long bone fractures in goats.
长骨骨折是常见的骨科病症。修复这些骨折有多种方法。当骨折处存在明显间隙时，骨移植就变得必要。然而，由于供体骨量不足以及供体部位的并发症，需要替代品。在兽医骨科中，蚶壳中的碳酸钙可作为骨生物材料。我们研究了其作为山羊股骨骨折骨生物材料修复的疗效。该研究纳入了10只体重8千克、年龄在12至13个月的健康成年雄性黑孟加拉山羊。研究包括对照组和治疗组。治疗组和对照组均采用髓内针固定8毫米右侧股骨干骨折。治疗组在骨折间隙注入2毫升骨糊，而对照组则保持骨折间隙为空。我们在第7天、第45天和第60天对所有山羊进行X线检查，随后进行大体和组织学检查。由于骨痂桥接，X线片显示治疗组的骨生长比对照组更快。大体检查表明，治疗组的骨折骨痂比对照组更大。组织病理学显示，与对照组相比，治疗组的骨形成更快，且包含更多的骨细胞、成骨细胞、破骨细胞和骨小梁。治疗组有更多的骨膜成骨细胞，而对照组有更多的成纤维细胞。这些结果表明，治疗组的成骨活性高于对照组。本研究证明了基于蚶壳的碳酸钙骨糊作为一种合成生物材料用于治疗山羊长骨骨折愈合的潜力。
