Department of Genetics, Maharshi Dayanand University, 124001, Rohtak, Haryana, India.
Extremophiles. 2024 Jul 22;28(3):33. doi: 10.1007/s00792-024-01353-3.
Carotenoids are a diverse group of pigments known for their broad range of biological functions and applications. This study delves into multifaceted potential of carotenoids extracted from Haloferax larsenii NCIM 5678 previously isolated from Pachpadra Salt Lake in Rajasthan, India. H. larsenii NCIM 5678 was able to grow up to OD 1.77 ± 0.03 with carotenoid concentration, 3.3 ± 0.03 µg/ml. The spectrophotometric analysis of carotenoid extract indicated the presence of three-fingered peak (460, 490 and 520 nm) which is a characteristic feature of bacterioruberin and its derivatives. The bacterioruberin was purified using silica gel column chromatography and thin layer chromatography. The carotenoid extract showed 12.3 ± 0.09 mm zone of growth inhibition with a minimum inhibitory concentration 546 ng/ml against indicator strain, H. larsenii HA4. The percentage antioxidant activity of carotenoid was found to be 84% which was higher as compared to commercially available ascorbic acid (56.74%). Thus, carotenoid extract from H. larsenii NCIM 5678 possesses unique attributes with compelling evidence of antimicrobial and antioxidant potential for the development of novel pharmaceuticals and nutraceuticals.
类胡萝卜素是一类具有广泛生物功能和应用的色素，其种类繁多。本研究深入探讨了先前从印度拉贾斯坦邦帕奇帕德拉盐湖中分离出的盐沼盐杆菌 NCIM 5678 中提取的类胡萝卜素的多方面潜在用途。盐沼盐杆菌 NCIM 5678 能够生长至 OD 1.77±0.03，类胡萝卜素浓度为 3.3±0.03µg/ml。类胡萝卜素提取物的分光光度分析表明存在三指峰（460、490 和 520nm），这是菌红菌素及其衍生物的特征。菌红菌素通过硅胶柱色谱和薄层色谱进行纯化。类胡萝卜素提取物对指示菌株盐沼盐杆菌 HA4 表现出 12.3±0.09mm 的抑菌生长抑制带，最小抑菌浓度为 546ng/ml。类胡萝卜素的抗氧化活性百分比为 84%，高于市售抗坏血酸（56.74%）。因此，盐沼盐杆菌 NCIM 5678 的类胡萝卜素提取物具有独特的属性，具有令人信服的抗菌和抗氧化潜力，可用于开发新型药物和营养保健品。
