Wang Jia, Zhao Yu-Jiao, Peng Hua-Sheng
School of Pharmacy, Anhui University of Chinese Medicine Hefei 230012, China.
School of Pharmacy, Anhui University of Chinese Medicine Hefei 230012, China Anhui Province Key Laboratory of Research & Development of Chinese Medicine Hefei 230012, China.
Zhongguo Zhong Yao Za Zhi. 2024 Jul;49(13):3484-3492. doi: 10.19540/j.cnki.cjcmm.20240414.101.
This study aims to reveal the differences in the species and relative content of metabolites in the leaf and root tuber of Fallopia multiflora and improve the comprehensive utilization rate of F. multiflora resources. The metabolites in the root tubers and leaves of F. multiflora were detected by widely targeted metabolomics based on ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS). The principal component analysis, hierarchical cluster analysis, and orthogonal partial least squares-discriminant analysis were carried out to screen the differential metabolites between the leaf and root tuber of F. multiflora. The result showed that a total of 1 942 metabolites in 15 categories were detected in the leaf and root tuber of F. multiflora, including 1 861 metabolites in the root tuber, 1 901 metabolites in the leaf, and 1 820 metabolites in both. The metabolites were mainly phenolic acids, flavonoids, amino acids and derivatives, and alkaloids. A total of 1 200 differential metabolites were screened out, accounting for 65.9% of the total metabolites. Among these differential metabolites, 813 and 387 showed higher content in the leaf and root tuber, respectively. Flavonoids were the metabolites with the largest number and the most significant differences between the leaf and root tuber, and stilbenes and anthraquinones as the main active compounds mainly existed in the root tuber. The KEGG enrichment results suggested that the differential metabolites were mainly enriched in flavonoid and flavonol biosynthesis pathways and linoleic acid metabolism pathway. This study discovered abundant metabolites in F. multiflora. The metabolites were similar but had great differences in the content between the leaf and root tuber. The research results provide theoretical guidance for the development and utilization of F. multiflora resources.
本研究旨在揭示何首乌叶和块根中代谢物的种类及相对含量差异，提高何首乌资源的综合利用率。采用基于超高效液相色谱-串联质谱（UPLC-MS/MS）的广泛靶向代谢组学方法检测何首乌块根和叶片中的代谢物。通过主成分分析、层次聚类分析和正交偏最小二乘法判别分析筛选何首乌叶和块根之间的差异代谢物。结果表明，何首乌叶和块根中共检测到15类1942种代谢物，其中块根中检测到1861种，叶片中检测到1901种，两者共有的有1820种。这些代谢物主要为酚酸、黄酮类、氨基酸及其衍生物和生物碱。共筛选出1200种差异代谢物，占总代谢物的65.9%。其中，813种和387种分别在叶片和块根中含量较高。黄酮类是叶和块根中数量最多、差异最显著的代谢物，而作为主要活性成分的芪类和蒽醌类主要存在于块根中。KEGG富集结果表明，差异代谢物主要富集在黄酮和黄酮醇生物合成途径以及亚油酸代谢途径中。本研究发现何首乌中含有丰富的代谢物，叶和块根中的代谢物种类相似，但含量差异较大。研究结果为何首乌资源的开发利用提供了理论指导。
