School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, PR China; Anhui Province Key Laboratory of Research & Development of Chinese Medicine, Hefei 230012, PR China.
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, PR China.
J Pharm Biomed Anal. 2021 Jun 5;200:114070. doi: 10.1016/j.jpba.2021.114070. Epub 2021 Apr 14.
Heshouwu, derived from root tubers of Fallopia multiflora (Thunb.) Harald., is a well-known herb used for millennia in traditional Chinese medicine. However, different forms of root tubers of Heshouwu have occurred in current Chinese herbal market and used in clinic, although it is still unknown whether their quality is consistent. In the present study, a mass spectrometry imaging and laser microdissection combined with UPLC-Q/TOF-MS were therefore used for the metabolite profiling on the whole and different parts of root tubers of F. multiflora and F. multiflora var. angulata. Our results suggested that the character of "woody heart" root tubers of F. multiflora was similar to that of F. multiflora var. angulata, but the latter had more phloem fibers and larger diameter vessel in the normal vascular bundle. Moreover, 140 compounds including stilbenes, anthraquinones, phenolic acids, naphthalenes, and other compounds were identified or putatively characterized from F. multiflora and F. multiflora var. angulata. Both unsupervised principal component analysis (PCA) and supervised Orthogonal Partial Least Squares Discrimination Analysis (OPLS-DA) multivariate statistics allowed discriminating F. multiflora and F. multiflora var. angulata. And a total of 32 potential markers were identified. The tissue-specific study indicated that the compounds in the phelloderm of F. multiflora and F. multiflora var. angulata were the most abundant. This is the first study on metabolite profiling and comparison of root tubers between F. multiflora and F. multiflora var. angulata, which would provide reasonable basis for further quality evaluation and safe medication of F. multiflora.
何首乌，来源于何首乌（Thunb.） Harald 的块根，是一种在传统中药中使用了数千年的著名草药。然而，目前的中草药市场上出现了不同形式的何首乌块根，并在临床上使用，尽管其质量是否一致仍不得而知。在本研究中，因此采用质谱成像和激光微切割结合 UPLC-Q/TOF-MS 对何首乌和狭叶何首乌的块根的整体和不同部位进行代谢物分析。我们的结果表明，何首乌的“木质心”块根的特征与狭叶何首乌相似，但后者在正常维管束中有更多的韧皮纤维和更大直径的导管。此外，从何首乌和狭叶何首乌中鉴定或推测出 140 种化合物，包括二苯乙烯类、蒽醌类、酚酸类、萘类和其他化合物。无监督主成分分析（PCA）和有监督正交偏最小二乘判别分析（OPLS-DA）多元统计允许区分何首乌和狭叶何首乌。并鉴定出 32 种潜在标志物。组织特异性研究表明，何首乌和狭叶何首乌的韧皮部中的化合物含量最丰富。这是何首乌和狭叶何首乌块根代谢组学分析和比较的首次研究，为进一步评价何首乌的质量和安全用药提供了合理依据。
