Department of Animal Science, Faculty of Agriculture, Ilam University, Ilam, Iran.
Department of Animal Science, Faculty of Agriculture and Environment, Arak University, Arak, Iran.
J Sci Food Agric. 2024 Dec;104(15):9324-9335. doi: 10.1002/jsfa.13755. Epub 2024 Jul 23.
Organic trace minerals (TM) offer superior nutritional benefits because of their stable structure, making their addition to broiler diets potentially beneficial during challenging periods such as aflatoxin B1 (AFB1) contamination. The present study evaluated the impacts of different replacement levels of inorganic TM (ITM) with advanced chelate technology-based TM (ACTM) on the growth performance, serum biochemical parameters, antioxidant indicators, and some inflammatory and immune parameters of broilers fed diets contaminated with AFB1. A 42-day experiment involved randomly assigning 1-day-old broiler chickens (n = 480) to one of five dietary treatments, each with six replicates. The treatments were as follows: (1) NC: basal diet without AFB1 and recommended ITM levels; (2) PC: basal diet with 0.5 mg kg AFB1 and recommended ITM levels; (3) TB: PC diet +1 g kg toxin binder; (4) ACTM50: replacement of ITM with 50% ACTM in the PC diet; and (5) ACTM100: replacement of ITM with 100% ACTM in the PC diet.
Compared with PC treatment, ACTM100 treatment resulted in increased (P < 0.05) body weight gain, serum zinc and glutathione concentrations, immunoglobulin Y level, antioxidant enzyme activities, and hepatic gene expression of nuclear factor erythroid 2-related factor 2, glutathione peroxidase-1, superoxide dismutase-1 and transforming growth factor beta 1. The ACTM100 group also exhibited decreased AFB1 residue in the liver and kidney, serum alanine transaminase activity and malondialdehyde concentration, and hepatic gene expression levels of nuclear factor-kappa B and interferon-gamma (P < 0.05). These values were comparable to those recorded in the TB and NC treatments.
In conclusion, completely replacing ITM with ACTM can benefit the metabolism and mitigate AFB1-induced immunotoxicity and oxidative damage in chickens by altering the mRNA expression of nuclear factor-kappa B and nuclear factor erythroid 2-related factor 2, and some genes downstream their signaling pathways. © 2024 Society of Chemical Industry.
有机微量元素 (TM) 因其稳定的结构而提供卓越的营养益处，因此在黄曲霉毒素 B1 (AFB1) 污染等具有挑战性的时期，将其添加到肉鸡日粮中可能具有益处。本研究评估了用先进螯合技术的 TM（ACTM）替代不同水平的无机 TM（ITM）对饲喂受 AFB1 污染日粮的肉鸡生长性能、血清生化参数、抗氧化指标以及一些炎症和免疫参数的影响。42 天的试验涉及将 1 日龄肉鸡（n=480）随机分配到五种日粮处理中的一种，每个处理有六个重复。处理如下：（1）NC：不含 AFB1 和推荐 ITM 水平的基础日粮；（2）PC：含 0.5mg/kg AFB1 和推荐 ITM 水平的基础日粮；（3）TB：PC 日粮+1g/kg 毒素结合剂；（4）ACTM50：PC 日粮中用 50%ACTM 替代 ITM；（5）ACTM100：PC 日粮中用 100%ACTM 替代 ITM。
与 PC 处理相比，ACTM100 处理增加了（P<0.05）体重增加、血清锌和谷胱甘肽浓度、免疫球蛋白 Y 水平、抗氧化酶活性和核因子红细胞 2 相关因子 2、谷胱甘肽过氧化物酶-1、超氧化物歧化酶-1 和转化生长因子β1 的肝基因表达。ACTM100 组还降低了肝脏和肾脏中的 AFB1 残留、血清丙氨酸转氨酶活性和丙二醛浓度以及核因子-κB 和干扰素-γ的肝基因表达水平（P<0.05）。这些值与 TB 和 NC 处理记录的值相当。
总之，完全用 ACTM 替代 ITM 可以通过改变核因子-κB 和核因子红细胞 2 相关因子 2 及其信号通路下游的一些基因的 mRNA 表达，改变肉鸡的代谢，减轻 AFB1 诱导的免疫毒性和氧化损伤。©2024 化学工业协会。
