Shanghai Xuhui Central Hospital, Zhongshan-Xuhui Hospital, and Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism, Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Chem Commun (Camb). 2024 Aug 13;60(66):8658-8669. doi: 10.1039/d4cc02319f.
Understansding complex biological systems requires the simultaneous characterization of a large number of interacting components in their native 3D environment with high spatial resolution. Highly-multiplexed Raman imaging is an emerging general strategy for detecting biomarkers with scalable multiplexity and ultra-sensitivity based on a series of stimulated Raman scattering (SRS) techniques. Here we review recent advances in highly-multiplexed Raman imaging and how they contribute to the technological revolution in 3D spatial biology, focusing on the developmental pathway from spectroscopy study to biotechnology invention. We envision highly-multiplexed Raman imaging is taking off, which will greatly facilitate our understanding in biological and medical research fields.
理解复杂的生物系统需要在其天然的 3D 环境中同时对大量相互作用的成分进行高空间分辨率的特征描述。高度多重化的拉曼成像技术是一种新兴的通用策略，可基于一系列受激拉曼散射（SRS）技术，以可扩展的多重性和超高灵敏度来检测生物标志物。在这里，我们回顾了高度多重化拉曼成像技术的最新进展，以及它们如何为 3D 空间生物学的技术革命做出贡献，重点介绍了从光谱研究到生物技术发明的发展途径。我们设想高度多重化的拉曼成像正在兴起，这将极大地促进我们在生物和医学研究领域的理解。
