College of Chemistry and Chemical Engineering, Inner Mongolia Key Laboratory of Fine Organic Synthesis, Institutes of Biomedical Sciences, Inner Mongolia University, Hohhot 010021, P. R. China.
Chem Commun (Camb). 2024 Aug 9;60(65):8484-8496. doi: 10.1039/d4cc02901a.
The rapid and sensitive detection of chemical compounds in body fluids and tissues is important for diagnosis of diseases and assessment of the effectiveness of treatment programs. Point-of-care (POC) sensors based on fluorescence signals have been widely used in the rapid detection of various infectious diseases. However, the aggregation-caused quenching phenomenon of conventional fluorescent probes limits the sensitivity and accuracy of fluorescent POC sensors. In this review, we first focus on aggregation-induced emission (AIE)-based POC detection for early diagnosis of diseases and then describe how to use mechanisms of AIE to improve the sensitivity of POC testing. This review gives a summary of the design mechanisms of AIE probes in AIE-based biosensors. Subsequently, it summarizes the design strategies of AIE-based POC sensors in the detection of ions, small molecules, nucleic acids, proteins, and whole entity (cells, bacteria, viruses, and exosomes), placing an emphasis on signal amplification. Finally, it gives an overview of AIE-based POC biosensors, including probes, instruments, and applications. We hope that this review will provide valuable guidance for further expanding the application of AIE luminogens in POC biosensors.
体液和组织中化学化合物的快速灵敏检测对于疾病诊断和治疗方案效果评估非常重要。基于荧光信号的即时检测（POC）传感器已广泛用于各种传染病的快速检测。然而，传统荧光探针的聚集猝灭现象限制了荧光 POC 传感器的灵敏度和准确性。在这篇综述中，我们首先关注基于聚集诱导发光（AIE）的 POC 检测在疾病的早期诊断中的应用，然后描述如何利用 AIE 机制来提高 POC 检测的灵敏度。本文综述了 AIE 探针在基于 AIE 的生物传感器中的设计机制。随后，总结了基于 AIE 的 POC 传感器在离子、小分子、核酸、蛋白质和整体实体（细胞、细菌、病毒和外泌体）检测中的设计策略，重点介绍了信号放大。最后，概述了基于 AIE 的 POC 生物传感器，包括探针、仪器和应用。我们希望这篇综述能为进一步拓展 AIE 发光体在 POC 生物传感器中的应用提供有价值的指导。
