Department of Epidemiology, School of Public Health, Shanxi Medical University, Taiyuan 030001, PR China.
National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, PR China.
Int J Syst Evol Microbiol. 2024 Jul;74(7). doi: 10.1099/ijsem.0.006465.
Six novel bacterial strains, designated N016, N017, N022, N028, N056, and N064, were isolated from soil sampled on the Qinghai-Tibet Plateau. Cells were aerobic, orange or yellow, globular or rod-shaped, non-motile, non-spore-forming, Gram-stain-positive, catalase-positive and oxidase-negative. All the isolates were salt-tolerant and could grow in the range of 4-42 °C. Results of phylogenomic analyses based on 16S rRNA gene sequences and core genomic genes showed that the three pairs of strains (N016/N017, N022/N028, and N056/N064) were closely related to the members of the genus , and clustered with , , and . The digital DNA-DNA hybridization and average nucleotide identity values of the six novel strains with other members of the genus were within the ranges of 18.7-53 % and 70.58-93.49 %, respectively, all below the respective recommended thresholds of 70.0 % and 95-96 %. The genomic DNA G+C content of the six strains ranged from 43.5 to 46.0 mol%. The major fatty acids of the six strains were -C, -C, and C 7 alcohol. The predominant polar lipids of strains N016, N022, and N056 were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. Menaquinones 7 and 8 were the respiratory quinones. The results of the above analyses indicated that the six strains represent three novel species of the genus , for which the names sp. nov. (type strain N016=GDMCC 1.4062=JCM 36224), sp. nov. (type strain N022=GDMCC 1.4063=JCM 36225), and sp. nov. (type strain N056=GDMCC 1.4064=JCM 36226) are proposed.
从青藏高原土壤中分离到 6 株新的细菌菌株,分别命名为 N016、N017、N022、N028、N056 和 N064。细胞呈需氧、橙色或黄色、球形或杆状、非运动、非孢子形成、革兰氏阳性、过氧化氢酶阳性和氧化酶阴性。所有分离株均具有耐盐性,可在 4-42°C 的范围内生长。基于 16S rRNA 基因序列和核心基因组基因的系统发育分析结果表明,三对菌株(N016/N017、N022/N028 和 N056/N064)与属内的成员密切相关,并与 、 、 和 聚类。六株新菌株与属内其他成员的数字 DNA-DNA 杂交和平均核苷酸同一性值分别为 18.7-53%和 70.58-93.49%,均低于各自 70.0%和 95-96%的推荐阈值。六株菌的基因组 DNA G+C 含量范围为 43.5-46.0mol%。六株菌的主要脂肪酸为 -C、-C 和 C 7 醇。菌株 N016、N022 和 N056 的主要极性脂为双磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺。menaquinones 7 和 8 是呼吸醌。上述分析结果表明,这 6 株菌代表了属的 3 个新种,分别命名为 sp. nov.(模式菌株 N016=GDMCC 1.4062=JCM 36224)、 sp. nov.(模式菌株 N022=GDMCC 1.4063=JCM 36225)和 sp. nov.(模式菌株 N056=GDMCC 1.4064=JCM 36226)。