Walls G A, Twentyman P R
Br J Cancer. 1985 Oct;52(4):505-13. doi: 10.1038/bjc.1985.221.
We have carried out a comparison of two different methods for cloning human lung cancer cells. The method of Courtenay & Mills (1978) generally gave higher plating efficiencies (PE) than the method of Carney et al. (1980). The number of colonies increased with incubation time in both methods and the weekly medium replenishment in the Courtenay method was advantageous for longer incubation times of several weeks. In the Courtenay method, the use of August rat red blood cells (RBC) and low oxygen tension were both found to be necessary factors for maximum plating efficiency. The usefulness of heavily irradiated feeder cells in improving PE is less certain; each cell type may have its own requirement.
我们对两种克隆人肺癌细胞的不同方法进行了比较。考特尼和米尔斯(1978年)的方法通常比卡尼等人(1980年)的方法具有更高的接种效率(PE)。在两种方法中,菌落数量均随培养时间增加,并且考特尼方法中每周更换培养基有利于长达数周的较长培养时间。在考特尼方法中,发现使用八月龄大鼠红细胞(RBC)和低氧张力都是获得最大接种效率的必要因素。严重辐照的饲养细胞在提高PE方面的有用性尚不确定;每种细胞类型可能有其自身的要求。