肺癌（主要是小细胞癌）患者细胞系的建立与鉴定。
Establishment and characterisation of cell lines from patients with lung cancer (predominantly small cell carcinoma).
作者信息
Baillie-Johnson H, Twentyman P R, Fox N E, Walls G A, Workman P, Watson J V, Johnson N, Reeve J G, Bleehen N M
出版信息
Br J Cancer. 1985 Oct;52(4):495-504. doi: 10.1038/bjc.1985.220.
Abstract
Tissue samples from 59 patients with lung cancer have been used to establish cell lines in culture. The primary diagnosis was small cell carcinoma in all except four. Most of the samples were of bone marrow but pleural effusions, lymph node biopsies and skin metastases were also included. The samples were usually split between HITES serum-free medium and HITES plus 2.5% foetal calf serum. A total of 19 cell lines were established and characterised. One line is large cell anaplastic lung carcinoma, four are B-lymphoblastoid and fourteen are small cell lung cancer. Considerable heterogeneity in gross morphology, neuroendocrine differentiation (by electron microscopy) and content of the enzyme L-dopa decarboxylase was seen. The use of HITES plus 2.5% foetal calf serum resulted in better establishment of cultures than did serum-free HITES.
摘要
来自59例肺癌患者的组织样本已被用于建立培养细胞系。除4例以外，所有患者的初步诊断均为小细胞癌。大多数样本来自骨髓，但也包括胸腔积液、淋巴结活检组织和皮肤转移灶。样本通常被分为两组，分别培养于无血清的HITES培养基和添加2.5%胎牛血清的HITES培养基中。共建立并鉴定了19个细胞系。其中1个细胞系为大细胞间变性肺癌，4个为B淋巴母细胞样细胞系，14个为小细胞肺癌细胞系。这些细胞系在大体形态、神经内分泌分化（通过电子显微镜观察）以及L-多巴脱羧酶含量方面存在显著异质性。与无血清的HITES培养基相比，添加2.5%胎牛血清的HITES培养基能更好地建立细胞培养体系。
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