Allalunis-Turner M J, Siemann D W
Br J Cancer. 1986 Oct;54(4):615-22. doi: 10.1038/bjc.1986.217.
Human epidermoid tumours (Co112, HEp3, A431, ME180) grown in nude mice were dissociated using four different enzyme cocktails: 0.025% collagenase, 0.05% pronase, 0.04% DNase; 0.1% protease IX; 0.14% trypsin, 0.04% DNase; 0.025% collagenase, 0.02% DNase. Using these different enzymatic procedures, the total cell yields, host to tumour cell ratios, plating efficiencies and cell cycle distribution profiles obtained from each tumour model were compared. For all tumours tested, enzyme cocktail 1 was the most effective in releasing the greatest total number of cells g-1 tumour. However, for each tumour the percentage of neoplastic cells recovered, the plating efficiency and the cell cycle distributions varied according to the enzyme cocktail used to dissociate the tumour. For example, for HEp3 tumours, the highest plating efficiency was achieved using enzyme cocktail 4, whereas for ME180 tumours, this enzyme cocktail produced the lowest plating efficiency. Further, the effect of lethally irradiated (HR) feeder cells on the plating efficiency of the various tumours was found to be influenced by the enzymes chosen to dissociate the tumours. These studies indicate that the choice of an enzyme dissociation technique may profoundly influence the results obtained using human tumour xenografts.
将生长于裸鼠体内的人表皮样肿瘤（Co112、HEp3、A431、ME180），使用四种不同的酶混合物进行解离：0.025%胶原酶、0.05%链霉蛋白酶、0.04%脱氧核糖核酸酶；0.1%蛋白酶IX；0.14%胰蛋白酶、0.04%脱氧核糖核酸酶；0.025%胶原酶、0.02%脱氧核糖核酸酶。采用这些不同的酶解方法，比较了从每个肿瘤模型获得的总细胞产量、宿主与肿瘤细胞比例、接种效率和细胞周期分布情况。对于所有测试的肿瘤，酶混合物1在释放每克肿瘤中最多数量的细胞方面最有效。然而，对于每个肿瘤，回收的肿瘤细胞百分比、接种效率和细胞周期分布根据用于解离肿瘤的酶混合物而有所不同。例如，对于HEp3肿瘤，使用酶混合物4可实现最高接种效率，而对于ME180肿瘤，该酶混合物产生的接种效率最低。此外，发现经致死剂量照射的（HR）饲养细胞对各种肿瘤接种效率的影响受所选用于解离肿瘤的酶的影响。这些研究表明，酶解离技术的选择可能会深刻影响使用人肿瘤异种移植获得的结果。
