Investigation of Operational Parameters for Nanoelectrokinetic Purification and Preconcentration.

This work reports on experimental investigations into the operational parameters of nanoelectrokinetic purification and preconcentration, especially utilizing on ion concentration polarization (ICP). ICP as a nanoscale electrokinetic phenomenon has demonstrated promising advances in various fields utilizing an ion depletion zone (IDZ) with a steep electric field gradient inside the ICP layer. However, the inevitable electrokinetic instability occurring within the IDZ has posed a challenge in operating the ICP system stably. To address the need for a stable and efficient ICP operation in various devices and applications, we propose an operational strategy along with conducted research to determine optimal operating ranges. In order to investigate the operational parameters, a unit voltage () is introduced as the threshold for initiating ICP. We examined the applicability of across various operating ranges to ensure its effectiveness and versatility. In ICP purification, we categorize three modes (steady, burst, and unsteady) based on IDZ expansion and stability under varying and flow rate conditions, presenting optimal operational conditions that minimize the voltage margin. In ICP preconcentration, a systematic investigation is conducted to observe the influence of background electrolyte concentration and voltage conditions on preconcentration efficiency, offering insights into the correlation between preconcentration factor, electrical conditions, and preconcentration time. Therefore, this research would contribute to the practical understanding of nanoelectrokinetics, providing insight into experimental designs. These findings are expected to offer valuable guidance to researchers aiming to utilize ICP's potential across a spectrum of applications, from purification to preconcentration, in the realm of micro/nanofluidic systems.