评估口腔潜在恶性疾病和恶性病变中唾液斑联蛋白的水平。

Evaluation of the levels of salivary paxillin in oral potentially malignant disorders and malignant lesions.

机构信息

Oral Medicine and Periodontology Department, Faculty of Dentistry, Cairo University, Cairo, Egypt.

Faculty of Medicine, Cairo University, Cairo, Egypt.

出版信息

BMC Oral Health. 2024 Jul 24;24(1):834. doi: 10.1186/s12903-024-04569-z.

DOI:10.1186/s12903-024-04569-z

PMID:39048985

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11267892/

Abstract

BACKGROUND

The scientific community has been particularly interested in oral squamous cell carcinoma (OSCC) because of the cancer's extremely high incidence and fatality rates worldwide. It has been proposed that paxillin is involved in certain malignancies as an oncogene. Additionally, several investigations have assessed paxillin expression and investigated its function in developing distinct human carcinomas, including squamous cell carcinoma. Furthermore, it was discovered that there is a strong link between paxillin levels and cancer progression and spread.

OBJECTIVE

This investigation was carried out to analyze and compare the salivary paxillin levels between oral potentially malignant disorders (OPMDs), OSCC and the healthy controls to assess its potential role as a biomarker of oral cancer aiming for early diagnosis and better prognosis of OSCC.

METHODS

Forty-five patients, ranging in age from thirty to seventy-five, were divided into three groups: fifteen patients with OPMDs, fifteen patients with OSCC, and fifteen controls. Paxillin was identified in saliva samples by using an ELISA kit.

RESULTS

Patients with OSCC and OPMDs have considerably greater salivary Paxillin levels than the healthy control group. The receiver operating curve (ROC) analysis was used in our study to distinguish patients with OPMDs from those with OSCC. The ROC curve constructed with the OPMDs group as the positives had lower sensitivity and area under the curve (AUC) values [100% and 1] than the ROC curve with the malignant group as the positives [93.3% and 0.997], respectively. Furthermore, ROC analysis performed between OPMDs group and the malignant group showed a specificity of 73.3% and a cut-off value ≥ 7.9 .

CONCLUSION

Paxillin can be considered a reliable biomarker for identifying and comparing OPMDs and OSCC cancerous changes.

GOV IDENTIFIER

NCT06154551- 4/12/2023.

摘要

背景

科学界对口腔鳞状细胞癌（OSCC）特别感兴趣，因为这种癌症在全球范围内的发病率和死亡率极高。有研究表明，桩蛋白作为癌基因参与某些恶性肿瘤的发生。此外，已有多项研究评估了桩蛋白的表达，并研究了其在不同人类癌中的作用，包括鳞状细胞癌。此外，还发现桩蛋白水平与癌症的进展和扩散之间存在很强的联系。

目的

本研究旨在分析和比较口腔潜在恶性疾病（OPMDs）、OSCC 和健康对照组之间的唾液桩蛋白水平，以评估其作为口腔癌生物标志物的潜在作用，旨在实现 OSCC 的早期诊断和更好的预后。

方法

将 45 名年龄在 30 至 75 岁之间的患者分为三组：15 名 OPMDs 患者、15 名 OSCC 患者和 15 名对照组。使用 ELISA 试剂盒检测唾液中的桩蛋白。

结果

OSCC 和 OPMDs 患者的唾液桩蛋白水平明显高于健康对照组。本研究采用受试者工作特征曲线（ROC）分析来区分 OPMDs 患者和 OSCC 患者。以 OPMDs 组为阳性的 ROC 曲线的敏感性和曲线下面积（AUC）值均低于以恶性组为阳性的 ROC 曲线[分别为 100%和 1] [分别为 93.3%和 0.997]。此外，OPMDs 组与恶性组之间的 ROC 分析显示，特异性为 73.3%，截断值≥7.9。