Aghili Seyedeh Sara, Zare Razieh, Jahangirnia Alireza
Student Research Committee, School of Dentistry, Shiraz University of Medical Sciences, Shiraz 71348-53734, Iran.
Department of Oral and Maxillofacial Pathology, School of Dentistry, Shiraz University of Medical Sciences, Shiraz 71348-53734, Iran.
Diagnostics (Basel). 2023 Jul 25;13(15):2476. doi: 10.3390/diagnostics13152476.
Paxillin is a cytoskeletal protein involved in the pathogenesis of several types of cancers. However, the roles of paxillin in epithelial dysplasia (ED), oral squamous cell carcinoma (OSCC), oral lichen planus with dysplasia (OLPD), hyperkeratosis (HK), and oral lichen planus (OLP) have remained unnoticed in the literature. This study aimed to evaluate its attainable functions in the pathogenesis and malignant transformation of potentially malignant oral epithelium and benign lesions.
In this retrospective cross-sectional study, paxillin expression was investigated in 99 tissue samples, including 18 cases of OSCC, 21 ED, 23 OLP, 21 OLPD, and 16 cases of HK. The tissue sections also underwent immunohistochemical paxillin staining using 3,3-diaminobenzidine (DAB) chromogen. The intensity, location, and percentage of staining were examined across all groups. Data were analyzed using the Shapiro-Wilk test, ANOVA, Pearson chi-square, Kruskal-Wallis, and Dunn's post hoc test.
The cytoplasmic percentage and intensity staining of Paxillin expression were evident in the central/suprabasal and basal/peripheral layers of all the obtained samples. The final staining score was significantly higher in OSCC and dysplasia compared to HK and OLP ( = 0.004). It was found that paxillin expression is associated with the grade of dysplastic samples ( < 0.001).
The present study provides evidence that paxillin may be involved in the pathogenesis of OSCC and the development and progression of dysplastic tissue, since the paxillin expression was higher than that of HK and OLP.
桩蛋白是一种细胞骨架蛋白，参与多种癌症的发病机制。然而，桩蛋白在上皮发育异常（ED）、口腔鳞状细胞癌（OSCC）、发育异常的口腔扁平苔藓（OLPD）、角化过度（HK）和口腔扁平苔藓（OLP）中的作用在文献中尚未得到关注。本研究旨在评估其在潜在恶性口腔上皮和良性病变的发病机制及恶性转化中可能发挥的作用。
在这项回顾性横断面研究中，对99个组织样本中的桩蛋白表达进行了研究，包括18例OSCC、21例ED、23例OLP、21例OLPD和16例HK。组织切片还使用3,3-二氨基联苯胺（DAB）显色剂进行了桩蛋白免疫组化染色。检查了所有组的染色强度、位置和百分比。数据采用Shapiro-Wilk检验、方差分析、Pearson卡方检验、Kruskal-Wallis检验和Dunn事后检验进行分析。
在所有获得的样本的中央/基底上层和基底/外周层中，桩蛋白表达的细胞质百分比和强度染色均很明显。与HK和OLP相比，OSCC和发育异常中的最终染色评分显著更高（P = 0.004）。发现桩蛋白表达与发育异常样本的分级相关（P < 0.001）。
本研究提供了证据表明，由于桩蛋白表达高于HK和OLP，桩蛋白可能参与OSCC的发病机制以及发育异常组织的发生和进展。
