International Iberian Nanotechnology Laboratory (INL), Braga, Portugal.
Translational Medical Oncology Group (Oncomet), Health Research Institute of Santiago de Compostela (IDIS), University Hospital of Santiago de Compostela (SERGAS), Santiago de Compostela, Spain.
Lab Chip. 2024 Aug 20;24(17):4028-4038. doi: 10.1039/d4lc00364k.
This paper describes the development, design and characterization of a resistive pulse sensing (RPS) system for the analysis of size distributions of extracellular vesicles (EVs). The system is based on microfluidic chips fabricated using soft-lithography and operated in pressure-driven mode. This fabrication approach provided reproducible pore dimensions and the best performing chip design enabled, without calibration, sizing of both 252 nm and 460 nm test particles within 8% of theoretically calculated values, based on the size specifications provided by suppliers. The number concentration measurement had higher variations and without calibration provided estimates within an order of magnitude, for sample concentrations across 4 orders of magnitude. The RPS chips could also measure successfully EVs and other biological nanoparticles in purified samples from cell culture media and human serum. A compact, fast and inexpensive RPS system based on this design could be an attractive alternative to current gold-standard techniques for routine characterization of EV samples.
本文描述了一种用于分析细胞外囊泡（EVs）大小分布的电阻脉冲传感（RPS）系统的开发、设计和特性。该系统基于软光刻技术制造的微流控芯片，以压力驱动模式运行。这种制造方法提供了可重复的孔径，而最佳性能的芯片设计使得无需校准，即可在 8%的理论计算值内对 252nm 和 460nm 测试粒子进行尺寸测量，这是基于供应商提供的尺寸规格。数量浓度测量的变化更大，且未经校准的情况下，在 4 个数量级的样品浓度范围内，提供的估计值在一个数量级内。RPS 芯片还可以成功测量细胞培养介质和人血清中纯化样品中的 EVs 和其他生物纳米颗粒。基于这种设计的紧凑、快速和廉价的 RPS 系统可能是当前 EV 样品常规特征分析的金标准技术的有吸引力的替代方案。
