The Department of Pharmacology and Molecular Sciences, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Methods Mol Biol. 2024;2823:1-10. doi: 10.1007/978-1-0716-3922-1_1.
It is now well accepted that individual cells within a population will respond to treatment of the same drug in a heterogenous manner. Recent advances have allowed, for the first time, the quantitative analysis of the proteomes of single human cells by mass spectrometry. A major focus of many groups, including our own, has been to use this emerging technology to rapidly identify subpopulations of cells with unique drug response and adaptation methods. While the technology in single-cell proteomics today is progressing at a truly staggering rate, we will detail our current methods for applying highly multiplexed single-cell proteomics to drug treatment studies.
现在人们普遍认为，同一药物处理同一群体中的个体细胞时，其反应方式是不均匀的。最近的进展使得通过质谱法对单个人类细胞的蛋白质组进行定量分析成为可能。包括我们在内的许多研究小组的主要重点一直是利用这项新兴技术快速识别具有独特药物反应和适应方法的细胞亚群。虽然单细胞蛋白质组学的技术目前正以惊人的速度发展，但我们将详细介绍我们目前将高通量单细胞蛋白质组学应用于药物治疗研究的方法。
