Organelle Architecture and Dynamics and 2 Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany.
J Cell Biol. 2010 Aug 23;190(4):491-500. doi: 10.1083/jcb.201004052.
The global analysis of protein composition, modifications, and dynamics are important goals in cell biology. Mass spectrometry (MS)-based proteomics has matured into an attractive technology for this purpose. Particularly, high resolution MS methods have been extremely successful for quantitative analysis of cellular and organellar proteomes. Rapid advances in all areas of the proteomic workflow, including sample preparation, MS, and computational analysis, should make the technology more easily available to a broad community and turn it into a staple methodology for cell biologists.
蛋白质组成、修饰和动态的全球分析是细胞生物学的重要目标。基于质谱（MS）的蛋白质组学已发展成为一种有吸引力的技术。特别是，高分辨率 MS 方法在细胞和细胞器蛋白质组的定量分析方面取得了巨大成功。蛋白质组学工作流程的所有领域都取得了快速进展，包括样品制备、MS 和计算分析，这应该使该技术更容易被广泛的群体所接受，并将其转化为细胞生物学家的一种基础方法。
