College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, Zhengzhou, China.
Nanjing Institute of Animal Husbandry and Poultry Science, Nanjing, China.
PLoS One. 2024 Jul 25;19(7):e0305907. doi: 10.1371/journal.pone.0305907. eCollection 2024.
The mechanisms governing gene regulation in domestic Yuzhong pigeon breast muscle development remain largely elusive. Here, we conducted a comparative analysis using Iso-seq and RNA-seq data from domestic Yuzhong pigeons and European meat pigeons to uncover signaling pathways and genes involved in breast muscle development. The Iso-seq data from domestic Yuzhong pigeons yielded 131,377,075 subreads, resulting in 16,587 non-redundant high-quality full-length transcripts post-correction. Furthermore, utilizing pfam, CPC, PLEK, and CPAT, we predicted 5575, 4973, 2333, and 4336 lncRNAs, respectively. Notably, several genes potentially implicated in breast muscle development were identified, including tropomyosin beta chain, myosin regulatory light chain 2, and myosin binding protein C. KEGG enrichment analysis revealed critical signaling pathways in breast muscle development, spanning carbon metabolism, biosynthesis of amino acids, glycolysis/gluconeogenesis, estrogen signaling, PI3K-AKT signaling, protein processing in the endoplasmic reticulum, oxidative phosphorylation, pentose phosphate pathway, fructose and mannose metabolism, and tight junctions. These findings offer insights into the biological processes driving breast muscle development in domestic Yuzhong pigeon, contributing to our understanding of this complex phenomenon.
调控家鸽胸肌发育的基因调控机制在很大程度上仍难以捉摸。在这里，我们使用家鸽和欧洲肉鸽的 Iso-seq 和 RNA-seq 数据进行了比较分析，以揭示参与胸肌发育的信号通路和基因。家鸽的 Iso-seq 数据产生了 131,377,075 个子序列，经校正后得到 16,587 个非冗余的高质量全长转录本。此外，利用 pfam、CPC、PLEK 和 CPAT，我们分别预测了 5575、4973、2333 和 4336 个 lncRNA。值得注意的是，鉴定出了几个可能与胸肌发育相关的基因，包括原肌球蛋白β链、肌球蛋白调节轻链 2 和肌球蛋白结合蛋白 C。KEGG 富集分析揭示了胸肌发育中的关键信号通路，包括碳代谢、氨基酸生物合成、糖酵解/糖异生、雌激素信号、PI3K-AKT 信号、内质网蛋白加工、氧化磷酸化、戊糖磷酸途径、果糖和甘露糖代谢以及紧密连接。这些发现为家鸽胸肌发育的生物学过程提供了新的认识，有助于我们理解这一复杂现象。
