Laboratory for Regenerative Tissue Repair, Texas Children's Hospital, Houston, TX, USA; Center for Congenital Cardiac Research, Texas Children's Hospital, Houston, TX, USA; Division of Congenital Heart Surgery, Texas Children's Hospital, Houston, TX, USA; Department of Surgery, Baylor College of Medicine, Houston, TX, USA; Division of Pediatric Surgery, Department of Surgery, Texas Children's Hospital, Houston, TX, USA.
Tissue Cell. 2024 Oct;90:102467. doi: 10.1016/j.tice.2024.102467. Epub 2024 Jul 9.
This review article will cover the recent developments in the new evolving field of Purkinje bioengineering and the development of human Purkinje networks. Recent work has progressed to the point of a methodological and systematic process to bioengineer Purkinje networks. This involves the development of 3D models based on human anatomy, followed by the development of tunable biomaterials, and strategies to reprogram stem cells to Purkinje cells. Subsequently, the reprogrammed cells and the biomaterials are coupled to bioengineer Purkinje networks, which are then tested using a small animal injury model. In this article, we discuss this process as a whole and then each step separately. We then describe potential applications of bioengineered Purkinje networks and challenges in the field that need to be overcome to move this field forward. Although the field of Purkinje bioengineering is new and in a state of infancy, it holds tremendous potential, both for therapeutic applications and to develop tools that can be used for disease modeling.
这篇综述文章将涵盖浦肯野生物工程这一新兴领域的最新进展，以及人类浦肯野网络的发展。最近的工作已经进展到了可以对浦肯野网络进行生物工程的方法学和系统性的过程。这涉及到基于人体解剖结构开发 3D 模型，然后开发可调谐生物材料，并制定策略将干细胞重编程为浦肯野细胞。随后，将重编程的细胞和生物材料结合起来，以生物工程的方式构建浦肯野网络，然后使用小动物损伤模型对其进行测试。在本文中，我们将整体讨论这一过程，然后分别讨论每个步骤。接着，我们描述了生物工程化的浦肯野网络的潜在应用以及该领域需要克服的挑战，以推动该领域的发展。尽管浦肯野生物工程领域是一个新兴的、处于起步阶段的领域，但它具有巨大的潜力，既可以用于治疗应用，也可以用于开发可用于疾病建模的工具。
