Department of Biomedical Engineering, Columbia University, New York, New York.
Department of Cell Biology, State University of New York Downstate Medical Center, Brooklyn, New York.
Am J Physiol Gastrointest Liver Physiol. 2021 Jan 1;320(1):G1-G11. doi: 10.1152/ajpgi.00206.2020. Epub 2020 Nov 11.
Gastrointestinal disease burden continues to rise in the United States and worldwide. The development of bioengineering strategies to model gut injury or disease and to reestablish functional gut tissue could expand therapeutic options and improve clinical outcomes. Current approaches leverage a rapidly evolving gut bioengineering toolkit aimed at ) de novo generation of gutlike tissues at multiple scales for microtissue models or implantable grafts and ) regeneration of functional gut in vivo. Although significant progress has been made in intestinal organoid cultures and engineered tissues, development of predictive in vitro models and effective regenerative therapies remains challenging. In this review, we survey emerging bioengineering tools and recent methodological advances to identify current challenges and future opportunities in gut bioengineering for disease modeling and regenerative medicine.
胃肠道疾病负担在美国和全球范围内持续上升。开发生物工程策略来模拟肠道损伤或疾病，并重建功能性肠道组织，可以扩大治疗选择并改善临床结果。目前的方法利用了一个快速发展的肠道生物工程工具包，旨在)在多个尺度上从头生成类似于肠道的组织，用于微组织模型或可植入移植物，以及)在体内再生功能性肠道。尽管在肠类器官培养和工程组织方面取得了重大进展，但开发预测性体外模型和有效的再生疗法仍然具有挑战性。在这篇综述中，我们调查了新兴的生物工程工具和最近的方法学进展，以确定肠道生物工程在疾病建模和再生医学方面的当前挑战和未来机遇。
