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[体内实验中S-甲基甲硫氨酸对原核RNA的甲基化作用]

[Methylation of prokaryotic RNA by S-methyl methionine in in vivo experiments].

作者信息

Lebenka A Iu, Rachkus Iu A, Kanopkaĭte S I

出版信息

Prikl Biokhim Mikrobiol. 1979 Sep-Oct;15(5):693-8.

PMID:390532
Abstract

While cultivating the E. coli 113-3 strain on the mineral medium containing S-methyl-(methyl-3H)-methionine, the incorporation of methyl groups into 4S, 16S and 23S RNA proved to be over 5 times more effective as compared with the control, when L-(methyl-3H)-methionine acted as a donor of methyl groups. The ratio of methylated components has much in common and significant differences. All the types of RNA of E. coli grown in the mineral medium containing S-methyl methionine showed an increased ratio of m2G as compared with the corresponding types of RNA of E. coli after its cultivation in the methionine containing mineral medium.

摘要

在用含有S-甲基-(甲基-³H)-甲硫氨酸的矿物培养基培养大肠杆菌113 - 3菌株时,与以L-(甲基-³H)-甲硫氨酸作为甲基供体的对照相比,甲基掺入4S、16S和23S RNA的效率提高了5倍多。甲基化成分的比例有许多共同之处和显著差异。在含有S-甲基甲硫氨酸的矿物培养基中生长的大肠杆菌的所有类型RNA,与在含有甲硫氨酸的矿物培养基中培养后的大肠杆菌相应类型RNA相比,m²G的比例都有所增加。

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