Sharma Surendra Raj, Hussain Sabir, Choudhary Shailesh K, Commins Scott P, Karim Shahid
School of Biological, Environment and Earth Sciences, The University of Southern Mississippi, Hattiesburg, MS 39406, USA.
School of Biological, Environment and Earth Sciences, The University of Southern Mississippi, Hattiesburg, MS 39406, USA; Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong SAR, China.
Ticks Tick Borne Dis. 2024 Nov;15(6):102384. doi: 10.1016/j.ttbdis.2024.102384. Epub 2024 Jul 24.
Alpha-Gal Syndrome (AGS) is a delayed allergic reaction triggered by IgE antibodies targeting galactose-α-1,3-galactose (α-gal), prevalent in red meat. Its global significance has increased, with over 450,000 estimated cases in the United States alone. AGS is linked to tick bites, causing sensitization and elevated α-gal specific IgE levels. However, the precise mechanisms and tick intrinsic factors contributing to AGS development post-tick bites remain unclear. This study aims to characterize the alpha-gal conjugated lipid antigens in Amblyomma americanum (Am. americanum) salivary glands and saliva. Nanospray ionization mass spectrometry (NSI-MS) analysis revealed the identification of α-gal bound lipid antigens in Am. americanum saliva. Additionally, the activation of basophils by extracted alpha-gal bound lipids and proteins provides evidence of their antigenic capabilities.
α-半乳糖综合征（AGS）是一种由靶向半乳糖-α-1,3-半乳糖（α-半乳糖）的IgE抗体引发的迟发性过敏反应，常见于红肉中。其全球影响日益增加，仅在美国估计就有超过45万例病例。AGS与蜱虫叮咬有关，会导致致敏和α-半乳糖特异性IgE水平升高。然而，蜱虫叮咬后导致AGS发生的确切机制以及蜱虫的内在因素仍不清楚。本研究旨在鉴定美洲钝眼蜱（美洲蜱）唾液腺和唾液中的α-半乳糖共轭脂质抗原。纳米喷雾电离质谱（NSI-MS）分析显示在美洲蜱唾液中鉴定出了α-半乳糖结合脂质抗原。此外，提取的α-半乳糖结合脂质和蛋白质对嗜碱性粒细胞的激活证明了它们的抗原能力。
