Research Unit VEG-i-TEC, Department of Food Technology, Safety, and Health, Faculty of Bioscience Engineering, Ghent University, Campus Kortrijk, Kortrijk, Belgium.
Univ. Lille, CNRS, INRAE, Centrale Lille, UMR 8207 - UMET - Unité Matériaux et Transformations, Lille, France.
Biofouling. 2024 Sep;40(8):467-482. doi: 10.1080/08927014.2024.2380410. Epub 2024 Jul 25.
Understanding factors influencing biofilms aid in developing more effective elimination/prevention strategies. This study examined the effect of temperature (4 °C, 21 °C, 30 °C), materials (stainless steel 316 L with 2B and 2 R finishes, glass, and polypropylene), and slope (0°/horizontal or 90°/vertical) on mono- and dual-species biofilms using two strains and one strain. All biofilms were grown in 10% TSB for 24 h and analyzed using culture-based methods. Additionally, the architecture of monospecies biofilms was studied using fluorescence microscopy. Overall, showed higher biofilm formation potential (6.2 log CFU/cm) than (4.0 log CFU/cm). Temperature greatly influenced and varied for The slope predominantly influenced monospecies biofilms, with cell counts increasing by up to 2 log CFU/cm. Surface material had little impact on biofilm formation. The study highlights the varying effects of different parameters on multispecies biofilms and the importance of surface geometry.
了解影响生物膜的因素有助于制定更有效的消除/预防策略。本研究通过两种 株和一种 株，考察了温度（4°C、21°C、30°C）、材料（不锈钢 316L 具有 2B 和 2R 两种表面处理、玻璃和聚丙烯）和坡度（0°/水平或 90°/垂直）对单种和混合生物膜的影响。所有生物膜均在 10% TSB 中培养 24 小时，并采用基于培养的方法进行分析。此外，还使用荧光显微镜研究了单种生物膜的结构。总体而言， 株的生物膜形成能力（6.2 log CFU/cm）高于 株（4.0 log CFU/cm）。温度对 和 的影响很大。坡度主要影响 株的单种生物膜，细胞计数最多增加了 2 log CFU/cm。表面材料对生物膜形成的影响较小。该研究强调了不同参数对多物种生物膜的不同影响以及表面几何形状的重要性。
