Seres-Steinbach Anita, Szabó Péter, Bányai Krisztián, Schneider György
Department of Medical Microbiology and Immunology, Medical School, University of Pécs, H-7624 Pécs, Hungary.
Department of Geology and Meteorology, Faculty of Sciences, University of Pécs, Ifjúság str. 6, H-7624 Pécs, Hungary.
Foods. 2025 Jun 14;14(12):2097. doi: 10.3390/foods14122097.
is a foodborne pathogen with a high tolerance to a wide range of environmental conditions, making its control in the food chain a particular challenge. Essential oils have recently been considered as potential antilisterial agents. In this study, the antilisterial effects of 57 EOs were tested on 13 different . Thirty-seven EOs were found to be effective in a strain and temperature-dependent manner. At 37 °C, all EOs were effective against at least one strain of . However, at 14 °C and 23 °C, 12 EOs, such as Minth, Nutmeg, Neroli, Pepperminth, etc., became drastically ineffective. The efficacy of the EOs increased at the lowest temperature, as only four EOs, such as Dill seed, Juniper, lemon eucalyptus, and sandalwood, were found to be ineffective at 4 °C. Ajowan and thyme were the only EOs that were antibacterial against each strain at all temperatures tested (4, 14, 23, 37 °C). Biofilm-inhibition tests with 57 EOs, performed on polystyrene plates with different surface qualities and stainless steel, using 0.1% and 0.5% final concentrations, showed the outstanding inhibitory abilities of ajowan, geranium, Lime oil, melissa, palmarosa, rose geranium, sandalwood, and thyme. Fennel, lemon eucalyptus, and chamomile had the potential to inhibit biofilm formation without affecting live bacterial cell counts. Ajowan, geranium, thyme, and palmarosa reduced the biofilm to the optical density of 0.0-0.08, OD: 0.0-0.075, 0.0-0.072, and 0.0-0.04, respectively, compared to the bacterium control 0.085-0.45. The mature antibiofilm eradication ability of the EOs revealed the outstanding features of ajowan, geranium Lime, melissa, palmarosa, rose geranium, and thyme by suppressing the established biofilm to one tenth. The different sensitivities of the isolates and the temperature-dependent antilisterial effect of the tested EOs have to be taken into account if an EO-based food preservation technology is to be implemented, as several become resistant to different EOs at medium temperature ranges such as 14 °C and 23 °C.
是一种食源性病原体，对多种环境条件具有高度耐受性，这使得在食物链中对其进行控制成为一项特殊挑战。精油最近被认为是潜在的抗李斯特菌剂。在本研究中，测试了57种精油对13种不同的……的抗李斯特菌作用。发现37种精油以菌株和温度依赖性方式有效。在37℃时，所有精油对至少一种……菌株有效。然而，在14℃和23℃时，12种精油，如薄荷、肉豆蔻、橙花、薄荷等，变得极无效。精油的功效在最低温度下有所提高，因为在4℃时仅发现4种精油，如莳萝籽、杜松、柠檬桉和檀香，无效。阿育吠陀和百里香是在所测试的所有温度（4、14、23、37℃）下对每种菌株都具有抗菌作用的仅有的精油。使用最终浓度为0.1%和0.5%，在具有不同表面质量的聚苯乙烯板和不锈钢上对57种精油进行生物膜抑制试验，结果显示阿育吠陀、天竺葵、柠檬油、香蜂草、玫瑰天竺葵、檀香和百里香具有出色的抑制能力。茴香、柠檬桉和洋甘菊有抑制生物膜形成的潜力，而不影响活细菌细胞计数。与细菌对照的光密度0.085 - 0.45相比，阿育吠陀、天竺葵、百里香和玫瑰天竺葵分别将生物膜降低至光密度0.0 - 0.08、OD：0.0 - 0.075、0.0 - 0.072和0.0 - 0.04。精油成熟的生物膜根除能力通过将已形成的生物膜抑制至十分之一，揭示了阿育吠陀、天竺葵、柠檬、香蜂草、玫瑰天竺葵和百里香的突出特性。如果要实施基于精油的食品保鲜技术，必须考虑分离株的不同敏感性以及所测试精油的温度依赖性抗李斯特菌作用，因为一些……在中等温度范围如14℃和23℃时对不同的精油产生抗性。
