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……中的多种类型核定位信号

Multiple types of nuclear localization signals in .

作者信息

Canela-Pérez Israel, Azuara-Liceaga Elisa, Cuéllar Patricia, Saucedo-Cárdenas Odila, Valdés Jesús

机构信息

Departamento de Bioquímica, CINVESTAV-México, Av. IPN 2508 colonia San Pedro Zacatenco, GAM, CDMX, 07360, Mexico.

Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, Mexico City, 03100, Mexico.

出版信息

Biochem Biophys Rep. 2024 Jul 4;39:101770. doi: 10.1016/j.bbrep.2024.101770. eCollection 2024 Sep.

DOI:10.1016/j.bbrep.2024.101770
PMID:39055170
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11269297/
Abstract

is a protozoan parasite that belongs to the Amoebozoa supergroup whose study related to the nucleocytoplasmic transport of proteins through the nucleus is poorly studied. In this work, we have performed predictions of the potential nuclear localization signals (NLS) corresponding to the proteome of 8201 proteins from annotated in the AmoebaDB database. We have found the presence of monopartite nuclear localization signals (MNLSs), bipartite nuclear localization signals (BNLSs), and non-canonical monopartite NLSs with lengths exceeding 20 amino acid residues. Additionally, we detected a new type of NLS consisting of multiple juxtaposed bipartite NLSs (JNLSs) that have not been described in any eukaryotic organism. Also, we have generated consensus sequences for the nuclear import of proteins with the NLSs obtained. Docking experiments between EhImportin α and an MNLS, BNLS, and JNLS outlined the interacting residues between the Importin and cargo proteins, emphasizing their putative roles in nuclear import. By transfecting HA-tagged protein constructs, we assessed the nuclear localization of MNLS (U1A and U2AF1), JMNLS (U2AF2), and non-canonical NLS (N-terminus of Pol ll) . Our data provide the basis for understanding the nuclear transport process in .

摘要

是一种原生动物寄生虫，属于变形虫超群，其与蛋白质通过细胞核的核质运输相关的研究较少。在这项工作中，我们对AmoebaDB数据库中注释的8201种蛋白质的蛋白质组对应的潜在核定位信号（NLS）进行了预测。我们发现了单部分核定位信号（MNLS）、双部分核定位信号（BNLS）以及长度超过20个氨基酸残基的非经典单部分NLS的存在。此外，我们检测到一种由多个并列的双部分NLS（JNLS）组成的新型NLS，这在任何真核生物中都未曾描述过。而且，我们利用获得的NLS生成了蛋白质核输入的共有序列。EhImportinα与MNLS、BNLS和JNLS之间的对接实验勾勒出了输入蛋白与货物蛋白之间的相互作用残基，强调了它们在核输入中的假定作用。通过转染带有HA标签的蛋白质构建体，我们评估了MNLS（U1A和U2AF1）、JMNLS（U2AF2）和非经典NLS（Pol ll的N端）的核定位。我们的数据为理解……中的核运输过程提供了基础。 （注：原文中“from annotated in the AmoebaDB database”表述不太准确，翻译时做了适当调整使其更通顺，但可能影响对原文准确含义的理解。“……”表示原文此处可能有缺失信息）

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/e3ba2036d5dc/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/e2a595076dd7/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/dd970e72fc60/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/37620850ae5a/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/1073904919f3/gr3a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/cbde853c4078/gr4a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/5e7f7ecccd61/gr5a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/293cd44bf173/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/a7a780cc5871/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/b0ec396e096b/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/3a292656de0f/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/da609460eaf4/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/e3ba2036d5dc/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/e2a595076dd7/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/dd970e72fc60/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/37620850ae5a/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/1073904919f3/gr3a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/cbde853c4078/gr4a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/5e7f7ecccd61/gr5a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/293cd44bf173/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/a7a780cc5871/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/b0ec396e096b/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/3a292656de0f/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/da609460eaf4/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f0e2/11269297/e3ba2036d5dc/gr11.jpg

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