Canela-Pérez Israel, López-Villaseñor Imelda, Mendoza Luis, Cevallos Ana María, Hernández Roberto
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, CP 04360, México.
Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad Universitaria, CP 04360, México.
Mol Biochem Parasitol. 2019 Apr;229:15-23. doi: 10.1016/j.molbiopara.2019.02.003. Epub 2019 Feb 14.
The nuclear import of proteins in eukaryotic cells is a fundamental biological process. While it has been analysed to different extents in model eukaryotic organisms, this event has rarely been studied in the early divergent protozoa of the order Kinetoplastida. The work presented here represents an overview of nuclear import in these important species of human pathogens. Initially, an in silico study of classical nuclear localization signals within the published nuclear proteomes of Trypanosoma brucei and Trypanosoma cruzi was carried out. The basic amino acids that comprise the monopartite and bipartite classical nuclear localization signals (cNLS) in trypanosomal proteins are similar to the consensus sequences observed for the nuclear proteins of yeasts, animals and plants. In addition, a summarized description of published studies that experimentally address the NLS of nuclear proteins in trypanosomatids is presented, and the clear occurrence of non-classical NLS (NLS that lack the consensus motifs of basic amino acids) in the analysed reports indicate a complex scenario for the types of receptors in these species. In general, the information presented here agrees with the hypothetical appearance of mechanisms for the recognition of nuclear proteins in early eukaryotic evolution.
真核细胞中蛋白质的核输入是一个基本的生物学过程。虽然在模式真核生物中已经对其进行了不同程度的分析，但在动质体目早期分化的原生动物中，这一过程很少被研究。本文介绍的工作概述了这些重要人类病原体物种中的核输入情况。首先，对布氏锥虫和克氏锥虫已发表的核蛋白质组中的经典核定位信号进行了计算机模拟研究。构成锥虫蛋白质中单一型和双分型经典核定位信号（cNLS）的碱性氨基酸与在酵母、动物和植物核蛋白中观察到的共有序列相似。此外，还总结了已发表的关于锥虫核蛋白NLS的实验研究描述，分析报告中明确出现的非经典NLS（缺乏碱性氨基酸共有基序的NLS）表明这些物种中受体类型的情况较为复杂。总体而言，本文提供的信息与早期真核生物进化中核蛋白识别机制的假设情况相符。
