Wang Yong-Hui, He Sheng-Hua, Huang Ji-Hong, Guo Wei-Yun, Gao Xue-Li, Li Guang-Hui
Food and Pharmacy College Xuchang University Xuchang People's Republic of China.
Collaborative Innovation Center of Functional Food Green Manufacturing Xuchang People's Republic of China.
Food Sci Nutr. 2024 Apr 18;12(7):5111-5120. doi: 10.1002/fsn3.4160. eCollection 2024 Jul.
Moderate non-covalent interaction of protein and polyphenols can improve the emulsifying property of protein itself. The corn protein hydrolysate (CPH) and tannic acid (TA) complex was successfully used to construct nanoemulsion for algal oil delivery. There has been no study on the feasibility of this nanoemulsion delivery system for other food functional components, for example, β-carotene (β-CE). CPH/TA complex-based nanoemulsion system for β-CE delivery was studied, focusing on the effect of β-CE content on the physicochemical stability of the nanoemulsions. The nanoemulsion delivery systems (dia. 150 nm) with low viscosity and good liquidity were easily fabricated by two-step emulsification. The nanoemulsions with high β-CE content (>71.5 μg/mL) significantly increased ( < .05) the emulsion droplet size. However, there was no significant ( > .05) effect of β-CE content on polydispersity index (PDI) and zeta potential of the nanoemulsions. The storage (30 days) experiment results demonstrated that the droplet size of the nanoemulsions with varying β-CE content increased slightly during storage. However, the PDI values showed a slightly decreasing trend. Zeta potentials of the nanoemulsions showed no noticeable change during storage. Moreover, after storage of 30 days, the retention ratios of β-CE were found to be up to 90%, which suggests an excellent protective effect for β-CE by the nanoemulsion systems. The CPH/TA complex stabilized nanoemulsions could aggregate in gastric condition, but the β-CE content did not have obvious effect on the digestive stability of the nanoemulsions. The CPH/TA complex could be employed as an emulsifier to construct a physicochemical stable nanoemulsion delivery system for lipophilic active components.
蛋白质与多酚之间适度的非共价相互作用能够改善蛋白质自身的乳化性能。玉米蛋白水解物（CPH）与单宁酸（TA）复合物已成功用于构建用于递送藻油的纳米乳液。尚未有关于这种纳米乳液递送系统用于其他食品功能成分（例如β-胡萝卜素（β-CE））可行性的研究。研究了基于CPH/TA复合物的用于递送β-CE的纳米乳液系统，重点关注β-CE含量对纳米乳液物理化学稳定性的影响。通过两步乳化法可轻松制备出具有低粘度和良好流动性的纳米乳液递送系统（直径150nm）。β-CE含量高（>71.5μg/mL）的纳米乳液显著增加（<0.05）了乳液滴尺寸。然而，β-CE含量对纳米乳液的多分散指数（PDI）和zeta电位没有显著（>0.05）影响。储存（30天）实验结果表明，不同β-CE含量的纳米乳液在储存期间滴尺寸略有增加。然而，PDI值呈略有下降的趋势。纳米乳液的zeta电位在储存期间没有明显变化。此外，储存30天后，发现β-CE的保留率高达90%，这表明纳米乳液系统对β-CE具有优异的保护作用。CPH/TA复合物稳定的纳米乳液在胃部环境中会聚集，但β-CE含量对纳米乳液的消化稳定性没有明显影响。CPH/TA复合物可作为乳化剂用于构建用于亲脂性活性成分的物理化学稳定的纳米乳液递送系统。
