Utilization of β-carotene in functional food products is limited due to chemical instability, lower water-solubility, and higher melting point. The present research was designed to formulate a nanoemulsion system for the effective delivery of β-carotene. β-Carotene was successfully incorporated into nanoemulsions using the ultrasonication method. During 60 days of storage, the droplet size of β-carotene-containing nanoemulsions varied from 112.36 to 133.9 nm at 4 °C and from 112.36 to 147.1 nm at 25 °C. The oxidation stability of olive oil was remarkably increased when incorporated into nanoemulsions. β-Carotene nanoemulsions remained stable under varying ionic strengths (50-400 mM), pH values (2-8), and freeze-thaw cycles (four). The values of turbidity and total color difference increased over time and at a higher temperature. Degradation of β-carotene was substantially slower in nanoemulsions, and the addition of antioxidants significantly increased the retention of β-carotene in nanoemulsions. These findings suggest that the ultrasonic homogenization method has potential for the preparation of β-carotene nanoemulsions with desirable properties. These nanoemulsions can be effectively used for the incorporation of β-carotene in the food and beverage industry.