叶片提取物通过下调活性氧（ROS）水平来抑制炭疽病的生长。
leaf extract inhibited anthracnose growth by downregulating reactive oxygen species (ROS) levels and .
机构信息
Central South University of Forestry and Technology, Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Changsha, Hunan, China.
Central South University of Forestry and Technology, Key Lab of Non-Wood Forest Products of State Forestry Administration, Changsha, Hunan, China.
出版信息
PeerJ. 2024 Jul 22;12:e17607. doi: 10.7717/peerj.17607. eCollection 2024.
BACKGROUND
is a predominant anthracnose species in , causing various adverse effects. Traditional intercropping with may enhance anthracnose resistance, but the mechanism remains elusive.
METHODS
We utilized UPLC-MS/MS and acid-base detection to identify the major antimicrobial alkaloid components in the leaf extract. Subsequently, by adding different concentrations of leaf extract for cultivating , with untreated as a control, we investigated the impact of the leaf extract, cell wall integrity, cell membrane permeability, MDA, and ROS content changes. Additionally, analysis of key pathogenic genes of confirmed that the leaf extract inhibits the growth of the fungus through gene regulation.
RESULTS
This study discovered the alkaloid composition of leaf extract by UPLC-MS/MS and acid-base detection, such as trigonelline, stachydrine, betaine, and O-Phosphocholine. leaf extract successfully penetrated mycelia, damaged cellular integrity, and increased ROS and MDA levels by 1.75 and 2.05 times respectively, thereby inhibiting proliferation. By analyzing the key pathogenic genes of , it was demonstrated that the antifungal function of leaf extract depends mainly on the regulation of and gene expression. Therefore, this study elucidates the mechanism of - intercropping in strengthening anthracnose resistance in , contributing to efficient cultivation.
背景
是引起各种不良影响的主要炭疽病物种。传统的与间作可能会增强炭疽病的抗性，但机制仍不清楚。
方法
我们利用 UPLC-MS/MS 和酸碱检测来鉴定柳叶提取物中的主要抗菌生物碱成分。然后，通过添加不同浓度的柳叶提取物来培养，以未处理的柳叶提取物作为对照，我们研究了柳叶提取物对细胞壁完整性、细胞膜通透性、MDA 和 ROS 含量变化的影响。此外，对关键致病基因的分析证实，柳叶提取物通过基因调控抑制真菌的生长。
结果
本研究通过 UPLC-MS/MS 和酸碱检测发现了柳叶提取物的生物碱成分，如葫芦巴碱、水苏碱、甜菜碱和 O-磷酸胆碱。柳叶提取物成功穿透菌丝，破坏细胞完整性，使 ROS 和 MDA 水平分别增加 1.75 倍和 2.05 倍，从而抑制真菌的增殖。通过分析关键致病基因，表明柳叶提取物的抑菌功能主要依赖于和基因表达的调控。因此，本研究阐明了间作增强的炭疽病抗性的机制，有助于的高效种植。
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