Turgut C T, Ozturk Civelek, Kotil T, Gungor S, Ozsoy Y, Dirican A, Okyar A, Yaltirik M
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of Istanbul, Istanbul, Turkey.
Department of Pharmacology, Faculty of Pharmacy, University of Bezmialem, Istanbul, Turkey.
Hippokratia. 2023 Apr-Jun;27(2):48-56.
In oral and maxillofacial surgery, hard tissue augmentation is provided by materials that accelerate the healing, act as a template for reconstructing bony defects, and stimulate bone production and growth. This study investigated the effects of biocompatible carriers containing active vitamin D and platelet-rich plasma (PRP) on bone defects created in rat calvaria.
This experimental animal study utilized fifty-two male Sprague Dawley rats divided into six groups and conducted histopathological, microtomographic, and biochemical comparisons by adding vitamin D, which has an accelerating effect on bone development. We examined the calvarial defects, sacrificing the rats in equal numbers (eight in each group and four to obtain PRP) at the first, second, fourth, and eighth weeks. The newly formed bone was assessed using histopathologic, microtomographic, and macroscopic methods as well as the biochemical analysis performed in the plasma samples. Expression of fibroblast growth factor 23 (FGF23), vitamin D receptor (VDR), and receptor activator of nuclear factor-kappa B (RANK), which determine bone formation, were investigated. The amounts of ossification, bone volume, and mineral densities were significantly higher in the fourth and eighth weeks as the biocompatible material was delivered with calcitriol.
The histological and macroscopic examinations revealed that the defect area shrank and was colonized with new cells in the "calcitriol + chitosan + PRP" group. The expression of RANK, FGF23, and VDR antibodies was more intense in the "calcitriol + chitosan + PRP" group than in other groups of the experiment and controls.
Active vitamin D, PRP, and chitosan formulation positively contributed to the repair of bone defects and induced remarkable clinical improvement. This new delivery approach could be promising for healing bone defects following surgical operations in hard bony tissues. HIPPOKRATIA 2023, 27 (2):48-56.
在口腔颌面外科中，硬组织增强是通过加速愈合、作为重建骨缺损模板并刺激骨生成和生长的材料来实现的。本研究调查了含有活性维生素D和富血小板血浆（PRP）的生物相容性载体对大鼠颅骨骨缺损的影响。
本实验动物研究使用了52只雄性Sprague Dawley大鼠，分为六组，并通过添加对骨骼发育有加速作用的维生素D进行组织病理学、显微断层扫描和生化比较。我们检查了颅骨缺损情况，在第一、第二、第四和第八周以相等数量（每组8只，4只用于获取PRP）处死大鼠。使用组织病理学、显微断层扫描和宏观方法以及血浆样本中的生化分析来评估新形成的骨。研究了决定骨形成的成纤维细胞生长因子23（FGF23）、维生素D受体（VDR）和核因子κB受体激活剂（RANK）的表达。随着生物相容性材料与骨化三醇一起递送，第四周和第八周的骨化量、骨体积和矿物质密度显著更高。
组织学和宏观检查显示，“骨化三醇+壳聚糖+PRP”组的缺损区域缩小，并被新细胞占据。“骨化三醇+壳聚糖+PRP”组中RANK、FGF23和VDR抗体的表达比实验和对照组的其他组更强烈。
活性维生素D、PRP和壳聚糖制剂对骨缺损的修复有积极作用，并带来了显著的临床改善。这种新的递送方法对于硬骨组织手术后骨缺损的愈合可能很有前景。《希波克拉底》2023年，27（2）：48 - 56。
