模块化聚酮合酶酮合酶与上游脱水酶协同作用以安装双键。
Modular polyketide synthase ketosynthases collaborate with upstream dehydratases to install double bonds.
机构信息
Department of Molecular Biosciences, The University of Texas at Austin, 100 E. 24th St., Austin, TX 78712, USA.
出版信息
Chem Commun (Camb). 2024 Aug 13;60(66):8712-8715. doi: 10.1039/d4cc03034f.
Abstract
A VMYH motif was determined to help ketosynthases in polyketide assembly lines select α,β-unsaturated intermediates from an equilibrium mediated by an upstream dehydratase. Alterations of this motif decreased ketosynthase selectivity within a model tetraketide synthase, most significantly when replaced by the TNGQ motif of ketosynthases that accept D-β-hydroxy intermediates.
摘要
一个 VMYH 基序被确定有助于聚酮装配线上的酮合酶从上游脱水酶介导的平衡中选择α，β-不饱和中间体。该基序的改变降低了模型四酮合酶中酮合酶的选择性，当被接受 D-β-羟基中间体的酮合酶的 TNGQ 基序取代时，影响最为显著。
Zotero 插件