通过工程化的聚酮合酶制备对映体对。
Preparative production of an enantiomeric pair by engineered polyketide synthases.
机构信息
Department of Molecular Biosciences, The University of Texas at Austin, 100 E. 24th St., Austin, TX 78712, USA.
出版信息
Chem Commun (Camb). 2021 Sep 11;57(70):8762-8765. doi: 10.1039/d1cc03073f. Epub 2021 Aug 11.
Abstract
Using the updated module boundary of polyketide assembly lines, modules from the pikromycin synthase were recombined into engineered synthases that furnish an enantiomeric pair of 2-stereocenter triketide lactones at >99% ee with yields up to 0.39 g per liter of E. coli K207-3 in shake flasks.
摘要
利用聚酮化合物装配线的更新模块边界，将 pikromycin 合酶的模块重新组合成工程合酶，可提供对映体纯的一对 2-立体中心三酮内酯，产率高达摇瓶中每升大肠杆菌 K207-3 的 0.39 克，ee 值大于 99%。
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