Department of Agricultural, Food and Nutritional Science, Faculty of Agricultural, Life and Environmental Sciences, University of Alberta, Edmonton, Alberta, Canada.
Department of Animal Sciences, Purdue University, West Lafayette, IN, USA.
Food Funct. 2024 Aug 12;15(16):8496-8509. doi: 10.1039/d4fo01813c.
Egg white protein ovomucin and its hydrolysates were previously reported to exhibit anti-inflammatory and anti-adhesive activities. However, their potential to regulate pathogen colonization and disease severity has not been fully characterized. To investigate the effects of ovomucin (OVM) and its hydrolysates including ovomucin-Protex 26L (OP) and -pepsin/pancreatin (OPP) on host resistance to pathogen infection, a well-documented colitis model in mice for attaching and effacing pathogens, , was used in the current study. C57Bl/6J female mice were fed on a basal diet supplemented with OVM or its hydrolysates for 3 weeks prior to the challenge, with the dietary treatments continued for seven days. Body weight was not affected throughout the experimental period. OP supplementation resulted in lower ( < 0.05) pathogen loads at 7 dpi. Attenuated colitis severity was observed in mice that received OVM and OP, as indicated by reduced colonic pathological scores and pro-inflammatory responses compared with the infected control group. In contrast, OPP consumption resulted in enhanced colonization and disease severity. Notably, reduced microbial diversity indices of the gut microbiota were observed in the OPP-supplemented mice compared with the OVM- and OP-supplemented groups. This study showed the potential of OVM and OP to alleviate the severity of colitis induced by infection while also suggesting the opposite outcome of OPP in mitigating enteric infection.
蛋清蛋白卵黏蛋白及其水解产物先前被报道具有抗炎和抗黏附活性。然而，它们调节病原体定植和疾病严重程度的潜力尚未得到充分表征。为了研究卵黏蛋白（OVM）及其水解产物（包括卵黏蛋白-Protex 26L（OP）和-胃蛋白酶/胰蛋白酶（OPP））对宿主抵抗病原体感染的影响，本研究使用了一种已被充分证明的黏附性和消蚀性病原体结肠炎模型。在攻毒前，C57Bl/6J 雌性小鼠用补充有 OVM 或其水解产物的基础饮食喂养 3 周，同时继续进行 7 天的饮食处理。整个实验期间，体重没有变化。OP 补充导致 7dpi 时病原体负荷较低（<0.05）。与感染对照组相比，接受 OVM 和 OP 治疗的小鼠结肠炎严重程度减轻，表现为结肠病理评分和促炎反应降低。相比之下，OPP 的消耗导致了 的定植和疾病严重程度的增加。值得注意的是，与 OVM 和 OP 补充组相比，OPP 补充组的肠道微生物群的微生物多样性指数降低。本研究表明 OVM 和 OP 具有缓解感染引起的结肠炎严重程度的潜力，同时也表明 OPP 在减轻肠道感染方面的相反作用。
