Department of Orthopaedics, Second Affiliated Hospital, Air Force Medical University, Xi'an, China.
Department of Orthopaedics, Xianyang Hospital of Yan'an University, Xianyang, China.
J Biomater Appl. 2024 Oct;39(4):332-342. doi: 10.1177/08853282241268683. Epub 2024 Jul 26.
The design and construction of a new and excellent synthetic graft is of great significance in the field of bone defect repair and reconstruction. In this study, a dopamine modified chitosan hydrogel doped with Cu ions with a mild photothermal effect was designed to provide a better microenvironment to advance the bone repair via promote the angiogenesis and osteogenesis. Characterizations showed the successful synthesis of the material while it also presented excellent biocompatibility and mild photothermal effect under the irradiation of near-infrared light. Further, it could enhance the angiogenesis of HUVECs cells through promoting the ability of migration and tube formation and enhance the osteogenic differentiation of MC3T3-E1 cells via increasing the content of vital osteogenic factors including Runx2, Col-1, OPN, OCN, OSX, etc. The in vivo experiment also testified that it could promote the bone defect repair in rat models. These results indicate the multifunctional hydrogel is an ideal material for the treatment of bone defects and has good clinical application potential.
设计和构建新型优质合成移植物在骨缺损修复和重建领域具有重要意义。在这项研究中，设计了一种具有温和光热效应的多巴胺修饰的铜离子掺杂壳聚糖水凝胶，通过促进血管生成和成骨作用，为骨修复提供更好的微环境。特性研究表明该材料成功合成，同时在近红外光照射下也表现出优异的生物相容性和温和的光热效应。此外，它可以通过促进迁移和管形成能力来增强 HUVECs 细胞的血管生成，并通过增加包括 Runx2、Col-1、OPN、OCN、OSX 等重要成骨因子的含量来增强 MC3T3-E1 细胞的成骨分化。体内实验也证明它可以促进大鼠模型的骨缺损修复。这些结果表明，多功能水凝胶是治疗骨缺损的理想材料，具有良好的临床应用潜力。
