Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, Taipei, Taiwan.
PhD Program in Clinical Drug Development of Herbal Medicine, College of Pharmacy, Taipei Medical University, Taipei, Taiwan.
Pharm Biol. 2024 Dec;62(1):621-633. doi: 10.1080/13880209.2024.2378011. Epub 2024 Jul 26.
Decne (Rosaceae), renowned for its therapeutic properties, is known to moisturize the lungs (removing dryness; relieving cough), clear heat (acting as an antipyretic; febrifuge) and aid in detoxification (relieving pyogenic inflammation; eliminating toxins). However, scientific evidence supporting its efficacy in wound healing is lacking.
This study investigated samples collected over a year to explore metabolite variations and their impact on skin wound-healing activities.
(PC) twigs and leaves were collected from the Matsu Islands, Taiwan, spanning 2018-2020. Extracts were prepared using 95% ethanol or water, and we assessed the chemical composition, total phenolic/triterpenoid contents and antioxidant properties. Metabolites were analysed via LC-MS/MS and molecular networking. Wound healing potential was evaluated on WS-1 cells through MTT and migration assays, and gene expression analyses, with tests including control (DMSO), compounds (3'-hydroxylbenzyl-4-hydroxybenzoate-4'--β-glucopyranoside) and (vanilloylcalleryanin) (100 µM), and a positive control (ascorbic acid, 100 µM) for 24 h.
Significant variations in extract compositions were observed based on the solvent used, with distinct metabolomic profiles in extracts collected during different months. Notably, compounds and showed no cytotoxic effects on human dermal fibroblast cells and significantly accelerated wound closure at 100 μM. A gene expression analysis indicated upregulation of wound healing-associated genes, including (matrix metalloproteinase-1) and (collagen, type 1, alpha 1).
This study reports the first evidence of PC compounds aiding wound healing. Utilizing Global Natural Products Social Molecular Networking (GNPS) and principal component analysis (PCA) approaches, we unveiled metabolomic profiles, suggesting the potential to expedite wound-healing.
棣棠（蔷薇科）以其治疗特性而闻名，具有润肺（润燥止咳）、清热（解热、退热）和解毒（化脓性炎症缓解、毒素消除）的功效。然而，缺乏科学证据支持其在伤口愈合方面的疗效。
本研究采集了一年多的样本，以探讨代谢物的变化及其对皮肤伤口愈合活性的影响。
2018 年至 2020 年，在台湾马祖岛采集棣棠（PC）的嫩枝和叶片。采用 95%乙醇或水提取，评估化学成分、总酚/三萜含量和抗氧化性能。采用 LC-MS/MS 和分子网络分析代谢物。通过 MTT 和迁移实验以及基因表达分析评估 WS-1 细胞的伤口愈合潜力，实验包括对照（DMSO）、化合物[3'-羟基苯甲基-4-羟基苯甲酸-4'--β-吡喃葡萄糖苷]和[香草酰基咖啡酰奎宁酸]（100 μM）以及阳性对照（抗坏血酸，100 μM），作用 24 小时。
根据溶剂的不同，提取物的组成有明显差异，不同月份采集的提取物具有不同的代谢组学特征。值得注意的是，化合物[3'-羟基苯甲基-4-羟基苯甲酸-4'--β-吡喃葡萄糖苷]和[香草酰基咖啡酰奎宁酸]在 100 μM 时对人真皮成纤维细胞无细胞毒性作用，且显著加速伤口闭合。基因表达分析表明，与伤口愈合相关的基因[基质金属蛋白酶-1（MMP-1）]和[胶原，类型 1，α 1（COL1A1）]表达上调。
本研究首次报道了棣棠化合物促进伤口愈合的证据。利用全球天然产物社会分子网络（GNPS）和主成分分析（PCA）方法，我们揭示了代谢组学特征，表明有可能加速伤口愈合。
