Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Mueang, Chiang Mai 50200, Thailand.
Research Center of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Int J Mol Sci. 2021 Dec 23;23(1):142. doi: 10.3390/ijms23010142.
Wound healing impairment due to a postponed, incomplete, or uncoordinated healing process has been a challenging clinical problem. Much research has focused on wound care, particularly on discovery of new therapeutic approaches for acute and chronic wounds. This study aims to evaluate the effect of the combination of quercetin and curcuminoids at three different ratios on the antimicrobial, antioxidant, cell migration and wound healing properties. The antioxidant activities of quercetin, curcuminoids and the mixtures were tested by DPPH and ABTS free radical scavenging assays. The disc diffusion method was performed to determine the antibacterial activities of quercetin, curcuminoids and the mixtures against and . The cytotoxicity and cell migratory enhancing effects of quercetin, curcuminoids and the mixtures against human dermal fibroblasts were investigated by MTT assay, scratch assay and Transwell migration assay, respectively. The results showed the synergism of the quercetin and curcuminoid combination to inhibit the growth of and , with the inhibition zone ranging from 7.06 ± 0.25 to 8.78 ± 0.38 mm, respectively. The DPPH free radical scavenging assay demonstrated that the combination of quercetin and curcuminoids yielded lower IC values (15.38-23.70 µg/mL) than curcuminoids alone (25.75 µg/mL). Quercetin and a 3:1 quercetin/curcuminoid mixture at non-toxic concentrations showed the ability to stimulate the migration of fibroblasts across the matrix, whereas only quercetin alone accelerated the wound closure of fibroblasts. In conclusion, the mixture of quercetin and curcuminoids at a 3:1 ratio was the best formulations for use in wound healing due to the antimicrobial, antioxidant and cell-migration-enhancing activities.
伤口愈合受损是一个具有挑战性的临床问题，其原因是愈合过程被推迟、不完整或不协调。许多研究都集中在伤口护理上，特别是探索急性和慢性伤口的新治疗方法。本研究旨在评估槲皮素和姜黄素在三种不同比例组合下对抑菌、抗氧化、细胞迁移和伤口愈合性能的影响。通过 DPPH 和 ABTS 自由基清除试验测试了槲皮素、姜黄素及其混合物的抗氧化活性。采用圆盘扩散法测定了槲皮素、姜黄素及其混合物对 和 的抗菌活性。通过 MTT 测定法、划痕试验和 Transwell 迁移试验分别研究了槲皮素、姜黄素及其混合物对人真皮成纤维细胞的细胞毒性和促迁移作用。结果表明，槲皮素和姜黄素组合具有协同作用，可抑制 和 的生长，抑制圈直径分别为 7.06 ± 0.25 至 8.78 ± 0.38mm。DPPH 自由基清除试验表明，槲皮素和姜黄素混合物的 IC 值（15.38-23.70μg/mL）低于姜黄素单独使用时的 IC 值（25.75μg/mL）。非毒性浓度的槲皮素和 3:1 的槲皮素/姜黄素混合物均能刺激成纤维细胞穿过基质迁移，而只有槲皮素能加速成纤维细胞的伤口闭合。总之，由于具有抑菌、抗氧化和促进细胞迁移的活性，槲皮素和姜黄素 3:1 的混合物是用于伤口愈合的最佳配方。
