Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, College of Light Industry and Food, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
Department of Entomology and Nematology and UCD Comprehensive Cancer Center, University of California, Davis, CA 95616, USA.
Biosensors (Basel). 2024 Jun 27;14(7):322. doi: 10.3390/bios14070322.
Patulin, an emerging mycotoxin with high toxicity, poses great risks to public health. Considering the poor antibody production in patulin immunization, this study focuses on the four-dimensional data-independent acquisition (4D-DIA) quantitative proteomics to reveal the immune response of patulin in rabbits. The rabbit immunization was performed with the complete developed antigens of patulin, followed by the identification of the immune serum. A total of 554 differential proteins, including 292 up-regulated proteins and 262 down-regulated proteins, were screened; the differential proteins were annotated; and functional enrichment analysis was performed. The differential proteins were associated with the pathways of metabolism, gene information processing, environmental information processing, cellular processes, and organismal systems. The functional enrichment analysis indicated that the immunization procedures mostly resulted in the regulation of biochemical metabolic and signal transduction pathways, including the biosynthesis of amino acid (glycine, serine, and threonine), ascorbate, and aldarate metabolism; fatty acid degradation; and antigen processing and presentation. The 14 key proteins with high connectivity included G1U9T1, B6V9S9, G1SCN8, G1TMS5, G1U9U0, A0A0G2JH20, G1SR03, A0A5F9DAT4, G1SSA2, G1SZ14, G1T670, P30947, P29694, and A0A5F9C804, which were obtained by the analysis of protein-protein interaction networks. This study could provide potential directions for protein interaction and antibody production for food hazards in animal immunization.
展青霉素是一种新兴的高毒性真菌毒素，对公众健康构成了巨大威胁。鉴于展青霉素免疫产生的抗体较差，本研究聚焦于四维非依赖性数据采集（4D-DIA）定量蛋白质组学，以揭示展青霉素在兔体中的免疫反应。用完全合成的展青霉素抗原对兔进行免疫接种，然后鉴定免疫血清。共筛选出 554 个差异蛋白，包括 292 个上调蛋白和 262 个下调蛋白；对差异蛋白进行注释，并进行功能富集分析。差异蛋白与代谢、基因信息处理、环境信息处理、细胞过程和生物系统途径有关。功能富集分析表明，免疫程序主要导致生化代谢和信号转导途径的调节，包括氨基酸（甘氨酸、丝氨酸和苏氨酸）、抗坏血酸和醛酸盐代谢、脂肪酸降解以及抗原加工和呈递的生物合成。具有高连接性的 14 个关键蛋白包括 G1U9T1、B6V9S9、G1SCN8、G1TMS5、G1U9U0、A0A0G2JH20、G1SR03、A0A5F9DAT4、G1SSA2、G1SZ14、G1T670、P30947、P29694 和 A0A5F9C804，这些蛋白是通过蛋白质-蛋白质相互作用网络分析获得的。本研究可为动物免疫中食品危害的蛋白质相互作用和抗体产生提供潜在方向。
