Cui Xuemei, Xu Xiangfei, Huang Pan, Bao Guolian, Liu Yan
Institute of Animal Husbandry and Veterinary Science, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China.
Pharmaceutics. 2022 Jul 8;14(7):1434. doi: 10.3390/pharmaceutics14071434.
Infectious respiratory diseases caused by () are seriously endangering the development of the rabbit industry in China. Unfortunately, no licensed vaccines are available for this pathogen. The present study was designed to determine whether the inactivated antigen formulated with vegetable oil adjuvant (named E515) which contains soybean oil, vitamin E, and ginseng saponins, functions as a safe and effective vaccine (E515-) against infection in rabbits. Based on local and systemic reactions, both the E515 adjuvant alone and the E515- vaccine exhibited good safety in rabbits. Immune response analysis implies that rabbits immunized with the E515- vaccine produced significantly higher, earlier, and longer-lasting specific antibody responses and activated Th1/Th2/Th17 cell responses than those immunized with the aluminum hydroxide (Alum)-adjuvanted vaccine (Alum-) or antigen alone. Moreover, the E515- vaccine effectively protected rabbits from infection. Additionally, integrated multi-omics analysis revealed that the immunoprotective effect of the E515- vaccine was achieved through upregulation of the complement and coagulation cascades and cell adhesion molecule (CAM) pathways, and the downregulation of the P53 pathway. Overall, these results indicate that the E515- vaccine is safe, elicits an efficient immune response and provides good protection against infection in rabbits. Thus, the E515-adjuvanted vaccine can be considered a promising candidate vaccine for preventing infection.
由（）引起的传染性呼吸道疾病严重危及中国兔产业的发展。不幸的是，目前尚无针对该病原体的许可疫苗。本研究旨在确定用含有大豆油、维生素E和人参皂苷的植物油佐剂配制的灭活抗原（命名为E515）是否能作为一种安全有效的疫苗（E515-）用于预防兔的（）感染。基于局部和全身反应，单独的E515佐剂和E515-疫苗在兔中均表现出良好的安全性。免疫反应分析表明，用E515-疫苗免疫的兔比用氢氧化铝（Alum）佐剂的（）疫苗（Alum-）或单独的（）抗原免疫的兔产生显著更高、更早且更持久的特异性抗体反应，并激活Th1/Th2/Th17细胞反应。此外，E515-疫苗有效地保护兔免受（）感染。另外，综合多组学分析显示，E515-疫苗的免疫保护作用是通过补体和凝血级联反应以及细胞粘附分子（CAM）途径的上调和P53途径的下调实现的。总体而言，这些结果表明E515-疫苗是安全的，能引发有效的免疫反应，并为兔提供良好的（）感染保护。因此，E515佐剂的（）疫苗可被认为是预防（）感染的一种有前景的候选疫苗。
