Kilgore Rebecca J, Guerra Trina, Beck Heather, Villamizar Gomez Andrea, Forstner Michael R J, Hahn Dittmar
The Tick-Borne Disease Research Laboratory, Department of Microbiology, Immunology, and Genetics, School of Biomedical Sciences, University of North Texas Health Science Center, Fort Worth, TX 76107, USA.
Department of Biology, Texas State University, 601 University Drive, San Marcos, TX 78666, USA.
Biology (Basel). 2024 Jun 30;13(7):489. doi: 10.3390/biology13070489.
The prevalence of was assessed in 117 triatomine insects from central Texas. The PCR-based results revealed in 59% of the insects (62 adults and eight nymphs), with overall prevalences of of 0% (0/9), 64% (11/17), 58% (10/17), 73% (30/41), and 57% (19/33) for the Bastrop, Caldwell, Gonzales, Guadalupe, and Hays counties, respectively. Analyses of 18S rRNA fragments confirmed in 81% of these samples. Vectors were identified as (35% of which 65% were positive for ), (21%, 43% positive), and (0.3%, 100% positive). Food sources were recovered from 29% of the insects. Raccoons were 53% of the blood meals (83% positive for ), while the remainder came from a variety of sources, including humans (33% positive), house geckos, Eastern woodrats, plain-bellied water snakes (50% positive), hispid cotton rats (0% positive), chickens (100% positive); Asian forest turtles, bison, and pigs (0% positive). The serendipitous detection of blood meal sources at known minimum distances from the collection of the vector insect enabled us to provide several instances where the insect foraging distance was greater than 400 m. These vector foraging distances are novel information that can assist in our understanding of the landscape dynamics for the spread of the pathogen.
对来自德克萨斯州中部的117只锥蝽昆虫进行了[病原体名称]的流行率评估。基于PCR的结果显示，59%的昆虫（62只成虫和8只若虫）感染了[病原体名称]，巴斯特罗普、考德威尔、冈萨雷斯、瓜达卢佩和海斯县的总体流行率分别为0%（0/9）、64%（11/17）、58%（10/17）、73%（30/41）和57%（19/33）。对18S rRNA片段的分析证实，这些样本中有81%感染了[病原体名称]。确定的病媒为[病媒种类1]（其中35%对[病原体名称]呈阳性）、[病媒种类2]（21%，43%呈阳性）和[病媒种类3]（0.3%，100%呈阳性）。在29%的昆虫体内发现了食物来源。浣熊占血餐的53%（对[病原体名称]呈阳性的比例为83%），其余来自多种来源，包括人类（33%呈阳性）、家壁虎、东部林鼠、平腹水蛇（50%呈阳性）、刚毛棉鼠（0%呈阳性）、鸡（100%呈阳性）；亚洲森林龟、野牛和猪（0%呈阳性）。在距病媒昆虫采集点已知最小距离处意外检测到血餐来源，这使我们能够提供几个病媒觅食距离大于400米的实例。这些病媒觅食距离是新信息，有助于我们了解病原体传播的景观动态。
