Nielsen David H, Koch Kyle, Roachell Walter, Delgado Bernardo, Bast Joshua
Entomological Sciences Division, Public Health Command-Central, Ft. Sam Houston, TX 78234, USA.
Entomolgy Program, Nebraska Department of Agriculture, 301 Centennial Mall South, Lincoln, NE 68509-4756, USA.
J Med Entomol. 2021 Nov 9;58(6):2519-2523. doi: 10.1093/jme/tjab122.
In 2017, a single adult Triatoma sanguisuga (LeConte) (Hemiptera: Reduviidae) was identified from Indian Cave State Park in Nemaha County, NE. The following summer, a single adult specimen was found in a 4-H insect collection at the Nebraska State Fair from Richardson County. A subsequent investigation found that these were collected from a residence in Richardson County and three more adults were collected in 2019. In 2020, the latter three kissing bugs were submitted to Public Health Command-Central's DoD Food Analysis and Diagnostic Laboratory (DOD FADL) for confirmatory species identification and diagnostic testing for Trypanosoma cruzi, the causative agent of Chagas disease. One specimen tested positive for T. cruzi using a real-time dual-target PCR screen followed by confirmatory dual-target traditional PCR. Based on these findings, a survey plan was developed for Richardson and surrounding counties in southeast Nebraska. In July of 2020, two adult and seven nymphs (multiple instars) of T. sanguisuga were collected in Richardson County, one of which tested positive for T. cruzi. This is the first record of an established population of T. sanguisuga and T. cruzi-infected kissing bugs in Nebraska.
2017年，在东北部内马哈县的印第安洞穴州立公园发现了一只成年红带锥蝽（LeConte）（半翅目：猎蝽科）。次年夏天，在内布拉斯加州立博览会理查森县的一个4-H昆虫收集品中发现了一只成年标本。随后的调查发现，这些标本采自理查森县的一处住宅，2019年又采集到另外三只成年红带锥蝽。2020年，后三只锥蝽被送交公共卫生司令部中部的国防部食品分析与诊断实验室（DOD FADL），以进行锥蝽物种鉴定及查加斯病病原体克氏锥虫的诊断检测。使用实时双靶点PCR筛查，随后进行双靶点传统PCR确认，有一只标本检测出克氏锥虫呈阳性。基于这些发现，为理查森县及内布拉斯加州东南部周边各县制定了一项调查计划。2020年7月，在理查森县采集到两只成年红带锥蝽和七只若虫（多龄期），其中一只检测出克氏锥虫呈阳性。这是内布拉斯加州红带锥蝽及感染克氏锥虫的锥蝽定殖种群的首次记录。
