Department of Clinical and Experimental Sciences, University of Brescia, 25123 Brescia, Italy.
Department of Molecular and Translational Medicine, University of Brescia, 25123 Brescia, Italy.
Cells. 2024 Jul 18;13(14):1210. doi: 10.3390/cells13141210.
Cancer cells require substantial amounts of energy and substrates for their metabolic hyperactivity, enabling the synthesis of new cells at the expense of healthy ones. Preliminary in vitro data suggest that a mix of free essential amino acids (EAA-mix) can promote cancer cell apoptosis by enhancing autophagy. This study aimed to confirm, both in vitro and in vivo, whether EAA intake could influence the development of colon cancer in mice. We investigated changes in cancer proliferation in CT26 cells treated with EAA-mix and in mice fed with EAA-rich modified diets (EAARD) as compared to those on a standard laboratory diet (StD). CT26 cells were injected subcutaneously (s.c.) or intraperitoneally (i.p.). After 21 days, tumors were removed and measured. In vitro data corroborated that EAA-mix impairs cancer growth by inducing apoptosis. In vivo data revealed that mice on StD developed significantly larger (s.c.) and more numerous (i.p.) cancers than those on EAARD. EAA administration appears to influence cancer cell survival with notable antiproliferative properties.
癌细胞需要大量的能量和底物来维持其代谢的过度活跃，从而以牺牲健康细胞为代价合成新的细胞。初步的体外数据表明，混合游离必需氨基酸（EAA 混合物）可以通过增强自噬来促进癌细胞凋亡。本研究旨在分别在体外和体内确认摄入 EAA 是否会影响小鼠结肠癌的发展。我们研究了 CT26 细胞在接受 EAA 混合物处理以及在喂食富含 EAA 的改良饮食（EAARD）的小鼠中与在标准实验室饮食（StD）上的癌症增殖变化。CT26 细胞通过皮下（s.c.）或腹腔内（i.p.）注射。21 天后，切除并测量肿瘤。体外数据证实，EAA 混合物通过诱导细胞凋亡来抑制癌症生长。体内数据显示，喂食 StD 的小鼠比喂食 EAARD 的小鼠形成的肿瘤明显更大（s.c.）且更多（i.p.）。EAA 的给予似乎会影响癌细胞的存活，具有显著的抗增殖特性。
