壳聚糖涂层在多孔钛基体上的电泳沉积

Electrophoretic Deposition of Chitosan Coatings on the Porous Titanium Substrate.

作者信息

Flesińska Julia, Szklarska Magdalena, Matuła Izabela, Barylski Adrian, Golba Sylwia, Zając Julia, Gawlikowski Maciej, Kurtyka Przemysław, Ilnicka Barbara, Dercz Grzegorz

机构信息

Institute of Materials Engineering, University of Silesia in Katowice, 75 Pułku Piechoty St. 1 A, 41-500 Chorzów, Poland.

Foundation of Cardiac Surgery Development, Institute of Heart Prostheses, 35a Wolności St., 41-800 Zabrze, Poland.

出版信息

J Funct Biomater. 2024 Jul 9;15(7):190. doi: 10.3390/jfb15070190.

Abstract

Medicine is looking for solutions to help implant patients recover more smoothly. The porous implants promote osteointegration, thereby providing better stabilization. Introducing porosity into metallic implants enhances their biocompatibility and facilitates osteointegration. The introduction of porosity is also associated with a reduction in Young's modulus, which reduces the risk of tissue outgrowth around the implant. However, the risk of chronic inflammation remains a concern, necessitating the development of coatings to mitigate adverse reactions. An interesting biomaterial for such modifications is chitosan, which has antimicrobial, antifungal, and osteointegration properties. In the present work, a porous titanium biomaterial was obtained by powder metallurgy, and electrophoretic deposition of chitosan coatings was used to modify its surface. This study investigated the influence of ethanol content in the deposition solution on the quality of chitosan coatings. The EPD process facilitates the control of coating thickness and morphology, with higher voltages resulting in thicker coatings and increased pore formation. Ethanol concentration in the solution affects coating quality, with higher concentrations leading to cracking and peeling. Optimal coating conditions (30 min/10 V) yield high-quality coatings, demonstrating excellent cell viability and negligible cytotoxicity. The GIXD and ATR-FTIR analysis confirmed the presence of deposited chitosan coatings on Ti substrates. The microstructure of the chitosan coatings was examined by scanning electron microscopy. Biological tests showed no cytotoxicity of the obtained materials, which allows for further research and the possibility of their use in medicine. In conclusion, EPD offers a viable method for producing chitosan-based coatings with controlled properties for biomedical applications, ensuring enhanced patient outcomes and implant performance.

摘要

医学领域正在寻找帮助植入患者更顺利康复的解决方案。多孔植入物可促进骨整合,从而提供更好的稳定性。在金属植入物中引入孔隙率可增强其生物相容性并促进骨整合。孔隙率的引入还与杨氏模量的降低有关,这降低了植入物周围组织生长的风险。然而,慢性炎症的风险仍然令人担忧,因此需要开发涂层来减轻不良反应。壳聚糖是一种用于此类改性的有趣生物材料,它具有抗菌、抗真菌和骨整合特性。在本工作中,通过粉末冶金获得了一种多孔钛生物材料,并使用壳聚糖涂层的电泳沉积来修饰其表面。本研究调查了沉积溶液中乙醇含量对壳聚糖涂层质量的影响。电泳沉积过程有助于控制涂层厚度和形态,较高的电压会导致涂层更厚且孔隙形成增加。溶液中的乙醇浓度会影响涂层质量,较高的浓度会导致涂层开裂和剥落。最佳涂层条件(30分钟/10伏)可产生高质量的涂层,显示出优异的细胞活力和可忽略不计的细胞毒性。掠入射X射线衍射(GIXD)和衰减全反射傅里叶变换红外光谱(ATR-FTIR)分析证实了钛基材上存在沉积的壳聚糖涂层。通过扫描电子显微镜检查了壳聚糖涂层的微观结构。生物学测试表明所获得的材料没有细胞毒性,这使得可以进行进一步的研究以及将其用于医学的可能性。总之,电泳沉积为生产具有可控性能的用于生物医学应用的壳聚糖基涂层提供了一种可行的方法,确保改善患者预后和植入物性能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1cce/11277708/d764fd40682e/jfb-15-00190-g001.jpg

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