用于引导性牙槽骨再生的新型半透性涂层在3D打印钛铌合金网片上的电泳沉积
Electrophoretic deposition of novel semi-permeable coatings on 3D-printed Ti-Nb alloy meshes for guided alveolar bone regeneration.
作者信息
Zhao Danlei, Dong Haoran, Niu Yuting, Fan Wenjie, Jiang Muqi, Li Ke, Wei Qingsong, Palin William M, Zhang Zhen
机构信息
Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan 430022, China; State Key Lab of Materials Processing and Die & Mould Technology, School of Materials, Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.
Department of Stomatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China; School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan 430022, China.
出版信息
Dent Mater. 2022 Feb;38(2):431-443. doi: 10.1016/j.dental.2021.12.026. Epub 2021 Dec 31.
OBJECTIVE
Guided bone regeneration (GBR) techniques use barrier membranes to augment the alveolar ridge for the site-specific growth of bone defects. However, current approaches using cast metal substructures exhibit poor adaptation to the surgical site and increased risk of infection. This study aimed to fabricate multi-functional coatings with 3D-printed porous titanium-niobium (Ti-Nb) alloy meshes to maintain space, prevent the ingrowth of fibroblasts and inhibit the colonization of bacteria for GBR.
METHODS
Ti-Nb alloy meshes were prepared by selective laser melting (SLM) and used as substrates for novel surface coatings. Porous chitosan (CS)/ gelatin (G)/ doxycycline (Dox) coatings were formed on the meshes using electrophoretic deposition (EPD) and freeze-drying. The process of EPD was characterized through Fourier transform infrared spectroscopy (FT-IR), zeta potential, and particle size analysis. The cytotoxicity of the coatings was evaluated through the culture of osteoblasts and immunostaining. The antibacterial activity of the coatings was tested using inhibition zone tests against Staphylococcus aureus (S. aureus) and scanning electron microscope (SEM). The inhibition of fibroblasts infiltration and nutrients transfer properties were analyzed using immunostaining and permeability tests.
RESULTS
High yield strength (567.5 ± 3.5 MPa) and low elastic modulus (65.5 ± 0.2 GPa) were achieved in Ti-Nb alloy bulk samples. The data of zeta potential, FT-IR and SEM indicated that porous spongy coatings were chemically bonded following EPD. In vitro analysis of CSGDox1 (containing Dox at 1 mg·mL) coating revealed its antibacterial effect and biocompatibility. Moreover, the CSGDox1 coating was proved to be effective for preventing the ingrowth of fibroblasts, whilst allowing the infiltration of nutrients.
SIGNIFICANCE
This study verified that the EPD of CSGDox coatings on the 3D-printed Ti-Nb meshes can maintain space, provide antibiotic release whilst maintaining a barrier against soft-tissue growth, which is essential for the success of GBR treatment.
目的
引导骨再生（GBR）技术使用屏障膜来增大牙槽嵴，以实现骨缺损部位特异性生长。然而，目前使用铸造金属子结构的方法对手术部位的适应性较差，且感染风险增加。本研究旨在制造具有3D打印多孔钛铌（Ti-Nb）合金网的多功能涂层，以维持空间、防止成纤维细胞长入并抑制细菌定殖，用于引导骨再生。
方法
通过选择性激光熔化（SLM）制备Ti-Nb合金网，并用作新型表面涂层的基底。使用电泳沉积（EPD）和冷冻干燥在网片上形成多孔壳聚糖（CS）/明胶（G）/多西环素（Dox）涂层。通过傅里叶变换红外光谱（FT-IR）、zeta电位和粒度分析对电泳沉积过程进行表征。通过成骨细胞培养和免疫染色评估涂层的细胞毒性。使用针对金黄色葡萄球菌（S. aureus）的抑菌圈试验和扫描电子显微镜（SEM）测试涂层的抗菌活性。使用免疫染色和渗透性测试分析对成纤维细胞浸润和营养物质转运特性的抑制作用。
结果
Ti-Nb合金块状样品实现了高屈服强度（567.5±3.5MPa）和低弹性模量（65.5±0.2GPa）。zeta电位、FT-IR和SEM数据表明，多孔海绵状涂层在电泳沉积后发生化学键合。对CSGDox1（含1mg·mL Dox）涂层的体外分析显示了其抗菌效果和生物相容性。此外，CSGDox1涂层被证明可有效防止成纤维细胞长入，同时允许营养物质渗透。
意义
本研究证实，在3D打印的Ti-Nb网片上进行CSGDox涂层的电泳沉积可维持空间、实现抗生素释放，同时保持对软组织生长的屏障作用，这对引导骨再生治疗的成功至关重要。
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