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通过氧化石墨烯增强溶胶-凝胶法制备的硅酸三钙的物理、抗菌和骨/牙源性特性用于牙髓治疗

Enhancing the Physical, Antimicrobial, and Osteo/Odontogenic Properties of a Sol-Gel-Derived Tricalcium Silicate by Graphene Oxide for Vital Pulp Therapies.

作者信息

Abdalla Mohamed Mahmoud, Nizami Mohammed Zahedul Islam, Rajasekar Vidhyashree, Basabrain Mohammed, Lung Christie Y K, Yiu Cynthia Kar Yung

机构信息

Paediatric Dentistry, Faculty of Dentistry, The University of Hong Kong, Hong Kong, China.

Dental Biomaterials, Faculty of Dental Medicine, Al-Azhar University, Cairo 11651, Egypt.

出版信息

J Funct Biomater. 2024 Jul 13;15(7):193. doi: 10.3390/jfb15070193.

DOI:10.3390/jfb15070193
PMID:39057314
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11278088/
Abstract

OBJECTIVES

This study developed a sol-gel tricalcium silicate/graphene oxide (TCS-GO) composite and examined its physicochemical properties, antimicrobial activity, and osteo/odontogenic effect on dental pulp stem cells.

METHODS

Tricalcium silicate was synthesized and combined with graphene oxide at three different concentrations, namely 0.02%, 0.04%, and 0.08% /, while tricalcium silicate and mineral trioxide aggregate served as controls. The setting time, compressive strength, pH, and calcium ion release of the composites were evaluated, as well as antimicrobial properties against Streptococcus mutans and Lactobacillus acidophilus. Additionally, the viability of dental pulp stem cells; apatite forming ability; and the gene expression of Alkaline phosphatase, Dentin sialophosphoprotein, and Runt-related transcription factor 2 were assessed.

RESULTS

TCS-GO (0.08%) showed a significantly shorter setting time and higher compressive strength when compared to MTA ( < 0.05). Additionally, tricalcium silicate and TCS-GO groups showed a higher release of Ca ions than MTA, with no significant difference in pH values among the different groups. TCS-GO (0.08%) also demonstrated a significantly stronger antimicrobial effect against Lactobacillus acidophilus compared to MTA ( < 0.05). ALP expression was higher in TCS-GO (0.08%) than MTA on days 3 and 7, while DSPP expression was higher in TCS-GO (0.08%) than MTA on day 3 but reversed on day 7. There was no significant difference in RUNX2 expression between TCS-GO (0.08%) and MTA on days 3 and 7.

CONCLUSIONS

The TCS-GO (0.08%) composite demonstrated superior physicochemical characteristics and antimicrobial properties compared to MTA. Moreover, the early upregulation of ALP and DSPP markers in TCS-GO (0.08%) indicates that it has the potential to promote and enhance the osteo/odontogenic differentiation of DPSCs.

摘要

目的

本研究制备了一种溶胶-凝胶硅酸三钙/氧化石墨烯（TCS-GO）复合材料，并研究了其物理化学性质、抗菌活性以及对牙髓干细胞的成骨/成牙本质作用。

方法

合成硅酸三钙并与三种不同浓度（即0.02%、0.04%和0.08%）的氧化石墨烯复合，同时以硅酸三钙和矿物三氧化物凝聚体作为对照。评估了复合材料的凝固时间、抗压强度、pH值和钙离子释放情况，以及对变形链球菌和嗜酸乳杆菌的抗菌性能。此外，还评估了牙髓干细胞的活力、磷灰石形成能力以及碱性磷酸酶、牙本质涎磷蛋白和 runt 相关转录因子 2 的基因表达。

结果

与MTA相比，TCS-GO（0.08%）的凝固时间显著缩短，抗压强度更高（P<0.05）。此外，硅酸三钙和TCS-GO组的钙离子释放量高于MTA，不同组之间的pH值无显著差异。与MTA相比，TCS-GO（0.08%）对嗜酸乳杆菌的抗菌作用也显著更强（P<0.05）。在第3天和第7天，TCS-GO（0.08%）中的碱性磷酸酶表达高于MTA，而在第3天，TCS-GO（0.08%）中的牙本质涎磷蛋白表达高于MTA，但在第7天则相反。在第3天和第7天，TCS-GO（0.08%）和MTA之间的RUNX2表达无显著差异。

结论

与MTA相比，TCS-GO（0.08%）复合材料具有优异的物理化学特性和抗菌性能。此外，TCS-GO（0.08%）中碱性磷酸酶和牙本质涎磷蛋白标志物的早期上调表明其有促进和增强牙髓干细胞成骨/成牙本质分化的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f85b/11278088/633eb6aae4a5/jfb-15-00193-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f85b/11278088/633eb6aae4a5/jfb-15-00193-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f85b/11278088/bd7982b3ce43/jfb-15-00193-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f85b/11278088/c91198e5db9c/jfb-15-00193-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f85b/11278088/6483d89c7693/jfb-15-00193-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f85b/11278088/987213d2e4c1/jfb-15-00193-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f85b/11278088/633eb6aae4a5/jfb-15-00193-g008.jpg

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