Dental Materials Science, Applied Oral Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, People's Republic of China.
Dental Biomaterials Department, Faculty of Dental Medicine, Al Azhar University, Cairo, Egypt.
J Biomed Mater Res B Appl Biomater. 2020 Jan;108(1):56-66. doi: 10.1002/jbm.b.34365. Epub 2019 Mar 28.
The aim of the current study was to synthesize a fast-setting ion-doped calcium silicate bioceramic by the sol-gel method and to characterize its in vitro apatite-forming ability and cell viability. Calcium silicate (CS), doped calcium silicate with zinc and magnesium, with Ca/Zn molar ratios of 6.7:1 (DCS1), and 4.5:1 (DCS2), were synthesized by the sol-gel method. Matreva white MTA (WMTA, Matreva, CA, Egypt) was used as a control. The synthesized powders were characterized by x-ray diffraction. Setting time was measured using the Gilmore needle indentation technique. The in vitro apatite-forming ability of the materials was evaluated by scanning electron microscope and energy dispersive X-ray. NIH3T3-E1 cells viability was tested using MTT assay. The ion release of Ca, Si, Zn, and Mg was measured using inductive coupled plasma-optical emission spectroscopy (ICP-OES). One-way ANOVA was used to analyze setting time results. The Tukey's HSD post hoc test was used to establish significance (p < 0.001). For nonparametric data, the Kruskal-Wallis H test with Dunn's correction for post hoc comparison was used (p < 0.05). CS, DCS1, and DCS2 showed a significant decrease in setting time 33 ± 1.63 min, 28 ± 1.63 min, and 41.75 ± 2.87 min, respectively, compared to WMTA 91 ± 3.16 min (p < 0.001). DCS1 showed the highest apatite-forming ability and cell viability compared to the other groups. Ca and Si ions release decreased in both DCS1 and DCS2. The physical and biological properties of CS can be successfully improved by the sol-gel synthesis and ions doping. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 108B:56-66, 2020.
本研究旨在通过溶胶-凝胶法合成一种快速凝固的掺离子硅酸钙生物陶瓷，并对其体外磷灰石形成能力和细胞活力进行表征。通过溶胶-凝胶法合成硅酸钙（CS）、掺锌和镁的硅酸钙，钙/锌摩尔比为 6.7:1（DCS1）和 4.5:1（DCS2）。采用 Matreva 白 MTA（WMTA，Matreva，CA，Egypt）作为对照。采用 X 射线衍射对合成粉末进行表征。采用吉尔莫针压痕技术测定凝固时间。采用扫描电子显微镜和能谱分析评价材料的体外磷灰石形成能力。采用 MTT 试验检测 NIH3T3-E1 细胞活力。采用电感耦合等离子体-光学发射光谱法（ICP-OES）测量 Ca、Si、Zn 和 Mg 的离子释放。采用单因素方差分析对凝固时间结果进行分析。采用 Tukey's HSD 事后检验确定显著性（p < 0.001）。对于非参数数据，采用 Kruskal-Wallis H 检验，并用 Dunn 校正进行事后比较（p < 0.05）。CS、DCS1 和 DCS2 的凝固时间分别显著缩短至 33±1.63min、28±1.63min 和 41.75±2.87min，与 WMTA 的 91±3.16min 相比（p < 0.001）。与其他组相比，DCS1 表现出最高的磷灰石形成能力和细胞活力。DCS1 和 DCS2 中 Ca 和 Si 离子的释放减少。通过溶胶-凝胶合成和离子掺杂，可以成功改善 CS 的物理和生物学性能。 © 2019 Wiley Periodicals, Inc. J 生物医学材料研究杂志 B：应用生物材料 108B：56-66，2020 年。
